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Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste

Author

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  • Laura Mejias

    (GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Aeris Tecnologías Ambientales S.L., Carrer Santa Rosa, 38, local, 08290 Cerdanyola del Vallès, Barcelona, Spain)

  • Alejandra Cerda

    (GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain)

  • Raquel Barrena

    (GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain)

  • Teresa Gea

    (GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain)

  • Antoni Sánchez

    (GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain)

Abstract

Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.

Suggested Citation

  • Laura Mejias & Alejandra Cerda & Raquel Barrena & Teresa Gea & Antoni Sánchez, 2018. "Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste," Sustainability, MDPI, vol. 10(7), pages 1-15, July.
    • Handle: RePEc:gam:jsusta:v:10:y:2018:i:7:p:2433-:d:157537
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    References listed on IDEAS

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    1. Noraziah Abu Yazid & Raquel Barrena & Dimitrios Komilis & Antoni Sánchez, 2017. "Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review," Sustainability, MDPI, vol. 9(2), pages 1-28, February.
    2. Sukumaran, Rajeev K. & Singhania, Reeta Rani & Mathew, Gincy Marina & Pandey, Ashok, 2009. "Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production," Renewable Energy, Elsevier, vol. 34(2), pages 421-424.
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    1. Brémond, Ulysse & Bertrandias, Aude & Loisel, Denis & Jimenez, Julie & Steyer, Jean-Philippe & Bernet, Nicolas & Carrere, Hélène, 2020. "Assessment of fungal and thermo-alkaline post-treatments of solid digestate in a recirculation scheme to increase flexibility in feedstocks supply management of biogas plants," Renewable Energy, Elsevier, vol. 149(C), pages 641-651.
    2. Eva Catalán & Antoni Sánchez, 2020. "Solid-State Fermentation (SSF) versus Submerged Fermentation (SmF) for the Recovery of Cellulases from Coffee Husks: A Life Cycle Assessment (LCA) Based Comparison," Energies, MDPI, vol. 13(11), pages 1-20, May.
    3. Ramón Verduzco-Oliva & Janet Alejandra Gutierrez-Uribe, 2020. "Beyond Enzyme Production: Solid State Fermentation (SSF) as an Alternative Approach to Produce Antioxidant Polysaccharides," Sustainability, MDPI, vol. 12(2), pages 1-14, January.
    4. Huanran Liu & Dan Zhang & Xia Zhang & Chuanzhi Zhou & Pei Zhou & Yuee Zhi, 2020. "Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method," Sustainability, MDPI, vol. 12(21), pages 1-12, October.

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