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Hydrolysis of pretreated rice straw by an enzyme cocktail comprising acidic xylanase from Aspergillus sp. for bioethanol production

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  • Thomas, Leya
  • Parameswaran, Binod
  • Pandey, Ashok

Abstract

The most crucial enzyme involved in xylan hydrolysis is endoxylanase which cleaves the internal glycosidic bonds of xylan. The aim of this work was to study the production of extracellular xylanase by a locally isolated strain of Aspergillus sp. under solid-state fermentation (SSF) and to evaluate the potential of the enzyme in enzymatic hydrolysis of pretreated rice straw. Xylanase production reached maximum with incubation period (96 h), moisture level (80%), inoculum size (3 × 106 spores/mL), pH (4.8), temperature (25 °C), carbon source (wheat bran) and nitrogen source (yeast extract). Under optimized conditions, xylanase production reached to 5059 IU/gds. Crude xylanase was used for supplementing the enzyme cocktail comprising cellulases (Zytex, India), β-glucosidase (In-house) and xylanase (In-house) for the saccharification of alkali-pretreated rice straw to get the maximum reducing sugar production. The cocktail containing the three enzymes resulted a maximum of 574.8 mg/g of total reducing sugars in comparison to 430.2 mg/g sugars by the cocktail without xylanase. These results proved that the crude xylanase preparation from Aspergillus sp. could be a potent candidate for the enzyme cocktail preparation for biomass hydrolysis in lignocellulosic bioethanol program.

Suggested Citation

  • Thomas, Leya & Parameswaran, Binod & Pandey, Ashok, 2016. "Hydrolysis of pretreated rice straw by an enzyme cocktail comprising acidic xylanase from Aspergillus sp. for bioethanol production," Renewable Energy, Elsevier, vol. 98(C), pages 9-15.
    • Handle: RePEc:eee:renene:v:98:y:2016:i:c:p:9-15
    DOI: 10.1016/j.renene.2016.05.011
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    Citations

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    Cited by:

    1. Ezeilo, Uchenna R. & Wahab, Roswanira Abdul & Mahat, Naji Arafat, 2020. "Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation," Renewable Energy, Elsevier, vol. 156(C), pages 1301-1312.
    2. da Rosa-Garzon, Nathália Gonsales & Laure, Hélen Julie & Rosa, José César & Cabral, Hamilton, 2019. "Fusarium oxysporum cultured with complex nitrogen sources can degrade agricultural residues: Evidence from analysis of secreted enzymes and intracellular proteome," Renewable Energy, Elsevier, vol. 133(C), pages 941-950.
    3. Bala, Anju & Singh, Bijender, 2019. "Development of an environmental-benign process for efficient pretreatment and saccharification of Saccharum biomasses for bioethanol production," Renewable Energy, Elsevier, vol. 130(C), pages 12-24.
    4. Mihajlovski, Katarina & Rajilić-Stojanović, Mirjana & Dimitrijević-Branković, Suzana, 2020. "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production," Renewable Energy, Elsevier, vol. 152(C), pages 627-633.
    5. Ghosh, Shiladitya & Chowdhury, Ranjana & Bhattacharya, Pinaki, 2017. "Sustainability of cereal straws for the fermentative production of second generation biofuels: A review of the efficiency and economics of biochemical pretreatment processes," Applied Energy, Elsevier, vol. 198(C), pages 284-298.
    6. Bañuelos, Jennifer A. & Velázquez-Hernández, I. & Guerra-Balcázar, M. & Arjona, N., 2018. "Production, characterization and evaluation of the energetic capability of bioethanol from Salicornia Bigelovii as a renewable energy source," Renewable Energy, Elsevier, vol. 123(C), pages 125-134.
    7. Noraziah Abu Yazid & Raquel Barrena & Dimitrios Komilis & Antoni Sánchez, 2017. "Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review," Sustainability, MDPI, vol. 9(2), pages 1-28, February.

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