IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v141y2019icp482-492.html
   My bibliography  Save this article

Enhanced bio-ethanol production from Chlorella sp. biomass by hydrothermal pretreatment and enzymatic hydrolysis

Author

Listed:
  • Ngamsirisomsakul, Marika
  • Reungsang, Alissara
  • Liao, Qiang
  • Kongkeitkajorn, Mallika Boonmee

Abstract

Microalgae are considered a third generation feedstock for biofuel production. Apart from lipid accumulation, some microalgae also accumulate carbohydrates which could be extracted and used as substrate for other biofuels production. This research aimed to assess potential of biomass of microalgae, Chlorella sp., for its potential as a feedstock for ethanol production. Response surface analysis was used to determine the conditions for pretreatment of microalgae biomass by acid-hydrothermal. The analysis suggested the use of 20% biomass in 1.5% sulfuric acid at 117 °C for 20 min as suitable for the pretreatment as it resulted in the maximum ethanol of 5.62 ± 0.16 g/L, which was 3.5 times higher compared with using untreated biomass. Enhanced ethanol production was investigated by hydrolyzing the pretreated slurry with α-amylase and glucoamylase. Using glucoamylase alone was enough for hydrolyzing the slurry to improve ethanol production. By using 25 Unit/galgae of glucoamylase, ethanol produced from the hydrolysate increased to 10.58 ± 0.34 g/L or 52.88 ± 1.69 mg/galgae. Enhancing ethanol production from Chlorella sp. biomass by hydrolyzing the acid-hydrothermal pretreated slurry with glucoamylase resulted in a total of 7.1 times increase in ethanol production from Chlorella sp. biomass as compared with the use of the biomass without any treatment.

Suggested Citation

  • Ngamsirisomsakul, Marika & Reungsang, Alissara & Liao, Qiang & Kongkeitkajorn, Mallika Boonmee, 2019. "Enhanced bio-ethanol production from Chlorella sp. biomass by hydrothermal pretreatment and enzymatic hydrolysis," Renewable Energy, Elsevier, vol. 141(C), pages 482-492.
  • Handle: RePEc:eee:renene:v:141:y:2019:i:c:p:482-492
    DOI: 10.1016/j.renene.2019.04.008
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148119304847
    Download Restriction: Full text for ScienceDirect subscribers only

    File URL: https://libkey.io/10.1016/j.renene.2019.04.008?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Naraharisetti, Pavan Kumar & Das, Probir & Sharratt, Paul N., 2017. "Critical factors in energy generation from microalgae," Energy, Elsevier, vol. 120(C), pages 138-152.
    2. Halim, Ronald & Harun, Razif & Danquah, Michael K. & Webley, Paul A., 2012. "Microalgal cell disruption for biofuel development," Applied Energy, Elsevier, vol. 91(1), pages 116-121.
    3. Giorgos Markou & Irini Angelidaki & Elias Nerantzis & Dimitris Georgakakis, 2013. "Bioethanol Production by Carbohydrate-Enriched Biomass of Arthrospira (Spirulina) p latensis," Energies, MDPI, vol. 6(8), pages 1-14, August.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Debnath, Chandrani & Bandyopadhyay, Tarun Kanti & Bhunia, Biswanath & Mishra, Umesh & Narayanasamy, Selvaraju & Muthuraj, Muthusivaramapandian, 2021. "Microalgae: Sustainable resource of carbohydrates in third-generation biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 150(C).
    2. Deb, Dipanwita & Mallick, Nirupama & Bhadoria, P.B.S., 2021. "Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach," Renewable Energy, Elsevier, vol. 163(C), pages 1786-1801.
    3. Qaseem, Mirza Faisal & Shaheen, Humaira & Wu, Ai-Min, 2021. "Cell wall hemicellulose for sustainable industrial utilization," Renewable and Sustainable Energy Reviews, Elsevier, vol. 144(C).
    4. Kavitha, S. & Gajendran, T. & Saranya, K. & Selvakumar, P. & Manivasagan, V., 2021. "Study on consolidated bioprocessing of pre-treated Nannochloropsis gaditana biomass into ethanol under optimal strategy," Renewable Energy, Elsevier, vol. 172(C), pages 440-452.
    5. Dhandayuthapani, K. & Kumar, P. Senthil & Chia, Wen Yi & Chew, Kit Wayne & Karthik, V. & Selvarangaraj, H. & Selvakumar, P. & Sivashanmugam, P. & Show, Pau Loke, 2022. "Bioethanol from hydrolysate of ultrasonic processed robust microalgal biomass cultivated in dairy wastewater under optimal strategy," Energy, Elsevier, vol. 244(PA).
    6. Shokrkar, Hanieh & Keighobadi, Amin, 2022. "Effect of fluid hydrodynamic situations on enzymatic hydrolysis of mixed microalgae: Experimental study and simulation," Energy, Elsevier, vol. 241(C).
    7. Jafari Olia, Mahroo Seyed & Azin, Mehrdad & Moazami, Nasrin, 2022. "Application of a statistical design to evaluate bioethanol production from Chlorella S4 biomass after acid - Thermal pretreatment," Renewable Energy, Elsevier, vol. 182(C), pages 60-68.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Arcigni, Francesco & Friso, Riccardo & Collu, Maurizio & Venturini, Mauro, 2019. "Harmonized and systematic assessment of microalgae energy potential for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 101(C), pages 614-624.
    2. Menegazzo, Mariana Lara & Fonseca, Gustavo Graciano, 2019. "Biomass recovery and lipid extraction processes for microalgae biofuels production: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 87-107.
    3. Al-Jabri, Hareb & Das, Probir & Khan, Shoyeb & AbdulQuadir, Mohammad & Thaher, Mehmoud Ibrahim & Hoekman, Kent & Hawari, Alaa H., 2022. "A comparison of bio-crude oil production from five marine microalgae – Using life cycle analysis," Energy, Elsevier, vol. 251(C).
    4. Goh, Brandon Han Hoe & Ong, Hwai Chyuan & Cheah, Mei Yee & Chen, Wei-Hsin & Yu, Kai Ling & Mahlia, Teuku Meurah Indra, 2019. "Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 59-74.
    5. Amarnath Krishnamoorthy & Cristina Rodriguez & Andy Durrant, 2022. "Sustainable Approaches to Microalgal Pre-Treatment Techniques for Biodiesel Production: A Review," Sustainability, MDPI, vol. 14(16), pages 1-30, August.
    6. Ayesha Aslam & Sumaira Rasul & Ali Bahadar & Nazia Hossain & Muhammad Saleem & Sabir Hussain & Lubna Rasool & Hamid Manzoor, 2021. "Effect of Micronutrient and Hormone on Microalgae Growth Assessment for Biofuel Feedstock," Sustainability, MDPI, vol. 13(9), pages 1-20, April.
    7. Yu, Kai Ling & Chen, Wei-Hsin & Sheen, Herng-Kuang & Chang, Jo-Shu & Lin, Chih-Sheng & Ong, Hwai Chyuan & Show, Pau Loke & Ng, Eng-Poh & Ling, Tau Chuan, 2020. "Production of microalgal biochar and reducing sugar using wet torrefaction with microwave-assisted heating and acid hydrolysis pretreatment," Renewable Energy, Elsevier, vol. 156(C), pages 349-360.
    8. Oncel, Suphi S., 2013. "Microalgae for a macroenergy world," Renewable and Sustainable Energy Reviews, Elsevier, vol. 26(C), pages 241-264.
    9. Nurdiawati, Anissa & Zaini, Ilman Nuran & Irhamna, Adrian Rizqi & Sasongko, Dwiwahju & Aziz, Muhammad, 2019. "Novel configuration of supercritical water gasification and chemical looping for highly-efficient hydrogen production from microalgae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 112(C), pages 369-381.
    10. Salam, Kamoru A. & Velasquez-Orta, Sharon B. & Harvey, Adam P., 2016. "A sustainable integrated in situ transesterification of microalgae for biodiesel production and associated co-product-a review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 65(C), pages 1179-1198.
    11. Henna Mohi ud din Wani & Chiu-Wen Chen & Chun-Yung Huang & Reeta Rani Singhania & Young Joon Sung & Cheng-Di Dong & Anil Kumar Patel, 2023. "Development of Bioactive Peptides Derived from Red Algae for Dermal Care Applications: Recent Advances," Sustainability, MDPI, vol. 15(11), pages 1-17, May.
    12. Saddam H. Al-lwayzy & Talal Yusaf & Raed A. Al-Juboori, 2014. "Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV) for Diesel Engines," Energies, MDPI, vol. 7(3), pages 1-23, March.
    13. D’Alessandro, Emmanuel B. & Antoniosi Filho, Nelson R., 2016. "Concepts and studies on lipid and pigments of microalgae: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 58(C), pages 832-841.
    14. Islam, Muhammad Aminul & Heimann, Kirsten & Brown, Richard J., 2017. "Microalgae biodiesel: Current status and future needs for engine performance and emissions," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1160-1170.
    15. Deb, Dipanwita & Mallick, Nirupama & Bhadoria, P.B.S., 2021. "Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach," Renewable Energy, Elsevier, vol. 163(C), pages 1786-1801.
    16. Wang, Songmei & Zhu, Johnny & Dai, Lingmei & Zhao, Xuebing & Liu, Dehua & Du, Wei, 2016. "A novel process on lipid extraction from microalgae for biodiesel production," Energy, Elsevier, vol. 115(P1), pages 963-968.
    17. Park, Ji-Yeon & Lee, Kyubock & Choi, Sun-A & Jeong, Min-Ji & Kim, Bohwa & Lee, Jin-Suk & Oh, You-Kwan, 2015. "Sonication-assisted homogenization system for improved lipid extraction from Chlorella vulgaris," Renewable Energy, Elsevier, vol. 79(C), pages 3-8.
    18. Wang, Linlin & Zhao, Runqi & Wang, Qi & Han, Zhaoze & Mao, Xian-zhong, 2022. "Novel bioreactor with inclined baffles in cost-efficiently increasing algal biomass and carbon fixation," Energy, Elsevier, vol. 247(C).
    19. Masoud Derakhshandeh & Tahir Atici & Umran Tezcan UN, 2019. "Lipid extraction from microalgae Chlorella and Synechocystis sp. using glass microparticles as disruption enhancer," Energy & Environment, , vol. 30(8), pages 1341-1355, December.
    20. Lim, Jackson Hwa Keen & Gan, Yong Yang & Ong, Hwai Chyuan & Lau, Beng Fye & Chen, Wei-Hsin & Chong, Cheng Tung & Ling, Tau Chuan & Klemeš, Jiří Jaromír, 2021. "Utilization of microalgae for bio-jet fuel production in the aviation sector: Challenges and perspective," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:141:y:2019:i:c:p:482-492. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.