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Identification and microbial production of a terpene-based advanced biofuel

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  • Pamela P. Peralta-Yahya

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory)

  • Mario Ouellet

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory)

  • Rossana Chan

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory)

  • Aindrila Mukhopadhyay

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory)

  • Jay D. Keasling

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory
    University of California)

  • Taek Soon Lee

    (Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, USA.
    Lawrence Berkeley National Laboratory)

Abstract

Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l−1. We produce bisabolene in Saccharomyces cerevisiae (>900 mg l−1), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels.

Suggested Citation

  • Pamela P. Peralta-Yahya & Mario Ouellet & Rossana Chan & Aindrila Mukhopadhyay & Jay D. Keasling & Taek Soon Lee, 2011. "Identification and microbial production of a terpene-based advanced biofuel," Nature Communications, Nature, vol. 2(1), pages 1-8, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1494
    DOI: 10.1038/ncomms1494
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    Cited by:

    1. Fernand, Francois & Israel, Alvaro & Skjermo, Jorunn & Wichard, Thomas & Timmermans, Klaas R. & Golberg, Alexander, 2017. "Offshore macroalgae biomass for bioenergy production: Environmental aspects, technological achievements and challenges," Renewable and Sustainable Energy Reviews, Elsevier, vol. 75(C), pages 35-45.
    2. Graham Rockwell & Nicholas J Guido & George M Church, 2013. "Redirector: Designing Cell Factories by Reconstructing the Metabolic Objective," PLOS Computational Biology, Public Library of Science, vol. 9(1), pages 1-15, January.
    3. Scaife, Mark A. & Merkx-Jacques, Alexandra & Woodhall, David L. & Armenta, Roberto E., 2015. "Algal biofuels in Canada: Status and potential," Renewable and Sustainable Energy Reviews, Elsevier, vol. 44(C), pages 620-642.
    4. Shihui Yang & Wei Wang & Hui Wei & Stefanie Van Wychen & Philip T. Pienkos & Min Zhang & Michael E. Himmel, 2016. "Comparison of Nitrogen Depletion and Repletion on Lipid Production in Yeast and Fungal Species," Energies, MDPI, vol. 9(9), pages 1-12, August.
    5. Xinyue Mu & Trent D. Evans & Fuzhong Zhang, 2024. "ATP biosensor reveals microbial energetic dynamics and facilitates bioproduction," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    6. Liu, Zihe & Moradi, Hamideh & Shi, Shuobo & Darvishi, Farshad, 2021. "Yeasts as microbial cell factories for sustainable production of biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 143(C).
    7. Ke-Na Feng & Yue Zhang & Mingfang Zhang & Yan-Long Yang & Ji-Kai Liu & Lifeng Pan & Ying Zeng, 2023. "A flavin-monooxygenase catalyzing oxepinone formation and the complete biosynthesis of vibralactone," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    8. Aivett Bilbao & Nathalie Munoz & Joonhoon Kim & Daniel J. Orton & Yuqian Gao & Kunal Poorey & Kyle R. Pomraning & Karl Weitz & Meagan Burnet & Carrie D. Nicora & Rosemarie Wilton & Shuang Deng & Ziyu , 2023. "PeakDecoder enables machine learning-based metabolite annotation and accurate profiling in multidimensional mass spectrometry measurements," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    9. Ulugbek Azimov & Victor Okoro & Hector H. Hernandez, 2021. "Recent Progress and Trends in the Development of Microbial Biofuels from Solid Waste—A Review," Energies, MDPI, vol. 14(19), pages 1-23, September.
    10. Das, Manali & Patra, Pradipta & Ghosh, Amit, 2020. "Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

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