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ATP biosensor reveals microbial energetic dynamics and facilitates bioproduction

Author

Listed:
  • Xinyue Mu

    (Washington University in St. Louis)

  • Trent D. Evans

    (Washington University in St. Louis)

  • Fuzhong Zhang

    (Washington University in St. Louis
    Division of Biological & Biomedical Sciences, Washington University in St. Louis
    Institute of Materials Science & Engineering, Washington University in St. Louis)

Abstract

Adenosine-5’-triphosphate (ATP), the primary energy currency in cellular processes, drives metabolic activities and biosynthesis. Despite its importance, understanding intracellular ATP dynamics’ impact on bioproduction and exploiting it for enhanced bioproduction remains largely unexplored. Here, we harness an ATP biosensor to dissect ATP dynamics across different growth phases and carbon sources in multiple microbial strains. We find transient ATP accumulations during the transition from exponential to stationary growth phases in various conditions, coinciding with fatty acid (FA) and polyhydroxyalkanoate (PHA) production in Escherichia coli and Pseudomonas putida, respectively. We identify carbon sources (acetate for E. coli, oleate for P. putida) that elevate steady-state ATP levels and boost FA and PHA production. Moreover, we employ ATP dynamics as a diagnostic tool to assess metabolic burden, revealing bottlenecks that limit limonene bioproduction. Our results not only elucidate the relationship between ATP dynamics and bioproduction but also showcase its value in enhancing bioproduction in various microbial species.

Suggested Citation

  • Xinyue Mu & Trent D. Evans & Fuzhong Zhang, 2024. "ATP biosensor reveals microbial energetic dynamics and facilitates bioproduction," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49579-1
    DOI: 10.1038/s41467-024-49579-1
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    References listed on IDEAS

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    1. Pamela P. Peralta-Yahya & Fuzhong Zhang & Stephen B. del Cardayre & Jay D. Keasling, 2012. "Microbial engineering for the production of advanced biofuels," Nature, Nature, vol. 488(7411), pages 320-328, August.
    2. Mark A. Lobas & Rongkun Tao & Jun Nagai & Mira T. Kronschläger & Philip M. Borden & Jonathan S. Marvin & Loren L. Looger & Baljit S. Khakh, 2019. "A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    3. Pamela P. Peralta-Yahya & Mario Ouellet & Rossana Chan & Aindrila Mukhopadhyay & Jay D. Keasling & Taek Soon Lee, 2011. "Identification and microbial production of a terpene-based advanced biofuel," Nature Communications, Nature, vol. 2(1), pages 1-8, September.
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