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Distribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors

Author

Listed:
  • Antonia Habich

    (Kiel University
    Max Planck Institute for Evolutionary Biology)

  • Verónica Chaves Vargas

    (Kiel University
    Max Planck Institute for Evolutionary Biology)

  • Luca A. Robinson

    (Imperial College London)

  • Luke P. Allsopp

    (Imperial College London)

  • Daniel Unterweger

    (Kiel University
    Max Planck Institute for Evolutionary Biology)

Abstract

Bacterial type VI secretion systems (T6SSs) are puncturing molecular machines that transport effector proteins to kill microbes, manipulate eukaryotic cells, or facilitate nutrient uptake. How and why T6SS machines and effectors differ within a species is not fully understood. Here, we applied molecular population genetics to the T6SSs in a global population of the opportunistic pathogen Pseudomonas aeruginosa. We reveal varying occurrence of up to four distinct T6SS machines. Moreover, we define conserved core T6SS effectors, likely critical for the biology of P. aeruginosa, and accessory effectors that can exhibit mutual exclusivity between strains. By ancestral reconstruction, we observed dynamic changes in the gain and loss of effector genes in the species’ evolutionary history. Our work highlights the potential importance of T6SS intraspecific diversity in bacterial ecology and evolution.

Suggested Citation

  • Antonia Habich & Verónica Chaves Vargas & Luca A. Robinson & Luke P. Allsopp & Daniel Unterweger, 2025. "Distribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors," Nature Communications, Nature, vol. 16(1), pages 1-23, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-54649-5
    DOI: 10.1038/s41467-024-54649-5
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    References listed on IDEAS

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    1. Alistair B. Russell & Rachel D. Hood & Nhat Khai Bui & Michele LeRoux & Waldemar Vollmer & Joseph D. Mougous, 2011. "Type VI secretion delivers bacteriolytic effectors to target cells," Nature, Nature, vol. 475(7356), pages 343-347, July.
    2. Alistair B. Russell & Michele LeRoux & Krisztina Hathazi & Danielle M. Agnello & Takahiko Ishikawa & Paul A. Wiggins & Sun Nyunt Wai & Joseph D. Mougous, 2013. "Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors," Nature, Nature, vol. 496(7446), pages 508-512, April.
    3. Chaya M. Fridman & Kinga Keppel & Motti Gerlic & Eran Bosis & Dor Salomon, 2020. "A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    4. Mikhail M. Shneider & Sergey A. Buth & Brian T. Ho & Marek Basler & John J. Mekalanos & Petr G. Leiman, 2013. "PAAR-repeat proteins sharpen and diversify the type VI secretion system spike," Nature, Nature, vol. 500(7462), pages 350-353, August.
    5. Shehryar Ahmad & Boyuan Wang & Matthew D. Walker & Hiu-Ki R. Tran & Peter J. Stogios & Alexei Savchenko & Robert A. Grant & Andrew G. McArthur & Michael T. Laub & John C. Whitney, 2019. "An interbacterial toxin inhibits target cell growth by synthesizing (p)ppApp," Nature, Nature, vol. 575(7784), pages 674-678, November.
    6. Jinshui Lin & Weipeng Zhang & Juanli Cheng & Xu Yang & Kaixiang Zhu & Yao Wang & Gehong Wei & Pei-Yuan Qian & Zhao-Qing Luo & Xihui Shen, 2017. "A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition," Nature Communications, Nature, vol. 8(1), pages 1-12, April.
    7. Martin Hagan & Genady Pankov & Ramses Gallegos-Monterrosa & David J. Williams & Christopher Earl & Grant Buchanan & William N. Hunter & Sarah J. Coulthurst, 2023. "Rhs NADase effectors and their immunity proteins are exchangeable mediators of inter-bacterial competition in Serratia," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    8. Daniel Unterweger & Sarah T. Miyata & Verena Bachmann & Teresa M. Brooks & Travis Mullins & Benjamin Kostiuk & Daniele Provenzano & Stefan Pukatzki, 2014. "The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition," Nature Communications, Nature, vol. 5(1), pages 1-9, May.
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