IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14888.html
   My bibliography  Save this article

A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition

Author

Listed:
  • Jinshui Lin

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
    Shaanxi Engineering & Technological Research Center for Conversation & Utilization of Regional Biological Resources, College of Life Sciences, Yan’an University)

  • Weipeng Zhang

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
    Hong Kong University of Science and Technology)

  • Juanli Cheng

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
    Shaanxi Engineering & Technological Research Center for Conversation & Utilization of Regional Biological Resources, College of Life Sciences, Yan’an University)

  • Xu Yang

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University)

  • Kaixiang Zhu

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University)

  • Yao Wang

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University)

  • Gehong Wei

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University)

  • Pei-Yuan Qian

    (Hong Kong University of Science and Technology)

  • Zhao-Qing Luo

    (Purdue Institute of Immunology, Purdue University)

  • Xihui Shen

    (State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University)

Abstract

Iron sequestration by host proteins contributes to the defence against bacterial pathogens, which need iron for their metabolism and virulence. A Pseudomonas aeruginosa mutant lacking all three known iron acquisition systems retains the ability to grow in media containing iron chelators, suggesting the presence of additional pathways involved in iron uptake. Here we screen P. aeruginosa mutants defective in growth in iron-depleted media and find that gene PA2374, proximal to the type VI secretion system H3 (H3-T6SS), functions synergistically with known iron acquisition systems. PA2374 (which we have renamed TseF) appears to be secreted by H3-T6SS and is incorporated into outer membrane vesicles (OMVs) by directly interacting with the iron-binding Pseudomonas quinolone signal (PQS), a cell–cell signalling compound. TseF facilitates the delivery of OMV-associated iron to bacterial cells by engaging the Fe(III)-pyochelin receptor FptA and the porin OprF. Our results reveal links between type VI secretion, cell–cell signalling and classic siderophore receptors for iron acquisition in P. aeruginosa.

Suggested Citation

  • Jinshui Lin & Weipeng Zhang & Juanli Cheng & Xu Yang & Kaixiang Zhu & Yao Wang & Gehong Wei & Pei-Yuan Qian & Zhao-Qing Luo & Xihui Shen, 2017. "A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition," Nature Communications, Nature, vol. 8(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14888
    DOI: 10.1038/ncomms14888
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14888
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14888?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dandan Wang & Lingfang Zhu & Xiangkai Zhen & Daoyan Yang & Changfu Li & Yating Chen & Huannan Wang & Yichen Qu & Xiaozhen Liu & Yanling Yin & Huawei Gu & Lei Xu & Chuanxing Wan & Yao Wang & Songying O, 2022. "A secreted effector with a dual role as a toxin and as a transcriptional factor," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Robert Zarnowski & Andrea Noll & Marc G. Chevrette & Hiram Sanchez & Ryley Jones & Hanna Anhalt & Jen Fossen & Anna Jaromin & Cameron Currie & Jeniel E. Nett & Aaron Mitchell & David R. Andes, 2021. "Coordination of fungal biofilm development by extracellular vesicle cargo," Nature Communications, Nature, vol. 12(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14888. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.