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Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells

Author

Listed:
  • Yuteng Weng

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Yanhuizhi Feng

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Zeyuan Li

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Shuyu Xu

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Di Wu

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Jie Huang

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Haicheng Wang

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

  • Zuolin Wang

    (Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology
    Tongji University)

Abstract

The initial fine-tuning processes are crucial for successful bone regeneration, as they guide skeletal stem cells through progenitor differentiation toward osteo- or chondrogenic fate. While fate determination processes are well-documented, the mechanisms preceding progenitor commitment remain poorly understood. Here, we identified a transcription factor, Zfp260, as pivotal for stem cell maturation into progenitors and directing osteogenic differentiation. Zfp260 is markedly up-regulated as cells transition from stem to progenitor stages; its dysfunction causes lineage arrest at the progenitor stage, impairing bone repair. Zfp260 is required for maintaining chromatin accessibility and regulates Runx2 expression by forming super-enhancer complexes. Furthermore, the PKCα kinase phosphorylates Zfp260 at residues Y173, S182, and S197, which are essential for its functional activity. Mutations at these residues significantly impair its functionality. These findings position Zfp260 as a vital factor bridging stem cell activation with progenitor cell fate determination, unveiling a element fundamental to successful bone regeneration.

Suggested Citation

  • Yuteng Weng & Yanhuizhi Feng & Zeyuan Li & Shuyu Xu & Di Wu & Jie Huang & Haicheng Wang & Zuolin Wang, 2024. "Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54640-0
    DOI: 10.1038/s41467-024-54640-0
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    References listed on IDEAS

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