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Optogenetic dissection of transcriptional repression in a multicellular organism

Author

Listed:
  • Jiaxi Zhao

    (University of California
    Harvard Medical School
    Brigham and Women’s Hospital)

  • Nicholas C. Lammers

    (University of California
    University of Washington)

  • Simon Alamos

    (University of California
    Joint BioEnergy Institute
    LBNL)

  • Yang Joon Kim

    (University of California)

  • Gabriella Martini

    (University of California)

  • Hernan G. Garcia

    (University of California
    University of California
    University of California
    University of California)

Abstract

Transcriptional control is fundamental to cellular function. However, despite knowing that transcription factors can repress or activate specific genes, how these functions are implemented at the molecular level has remained elusive, particularly in the endogenous context of developing animals. Here, we combine optogenetics, single-cell live-imaging, and mathematical modeling to study how a zinc-finger repressor, Knirps, induces switch-like transitions into long-lived quiescent states. Using optogenetics, we demonstrate that repression is rapidly reversible (~1 min) and memoryless. Furthermore, we show that the repressor acts by decreasing the frequency of transcriptional bursts in a manner consistent with an equilibrium binding model. Our results provide a quantitative framework for dissecting the in vivo biochemistry of eukaryotic transcriptional regulation.

Suggested Citation

  • Jiaxi Zhao & Nicholas C. Lammers & Simon Alamos & Yang Joon Kim & Gabriella Martini & Hernan G. Garcia, 2024. "Optogenetic dissection of transcriptional repression in a multicellular organism," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53539-0
    DOI: 10.1038/s41467-024-53539-0
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    References listed on IDEAS

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    2. Ralph Stadhouders & Guillaume J. Filion & Thomas Graf, 2019. "Transcription factors and 3D genome conformation in cell-fate decisions," Nature, Nature, vol. 569(7756), pages 345-354, May.
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