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Optogenetic control of nuclear protein export

Author

Listed:
  • Dominik Niopek

    (German Cancer Research Center (DKFZ)
    Synthetic Biology Group, Institute for Pharmacy and Biotechnology (IPMB), University of Heidelberg
    Center for Quantitative Analysis of Molecular and Cellular Biosystems (BioQuant), University of Heidelberg)

  • Pierre Wehler

    (German Cancer Research Center (DKFZ))

  • Julia Roensch

    (German Cancer Research Center (DKFZ))

  • Roland Eils

    (German Cancer Research Center (DKFZ)
    Synthetic Biology Group, Institute for Pharmacy and Biotechnology (IPMB), University of Heidelberg
    Center for Quantitative Analysis of Molecular and Cellular Biosystems (BioQuant), University of Heidelberg)

  • Barbara Di Ventura

    (Synthetic Biology Group, Institute for Pharmacy and Biotechnology (IPMB), University of Heidelberg
    Center for Quantitative Analysis of Molecular and Cellular Biosystems (BioQuant), University of Heidelberg)

Abstract

Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically encoded tag, which enables precise spatiotemporal control over the export of tagged proteins. A constitutively nuclear, chromatin-anchored LEXY variant expands the method towards light inhibition of endogenous protein export by sequestering cellular CRM1 receptors. We showcase the utility of LEXY for cell biology applications by regulating a synthetic repressor as well as human p53 transcriptional activity with light. LEXY is a powerful addition to the optogenetic toolbox, allowing various novel applications in synthetic and cell biology.

Suggested Citation

  • Dominik Niopek & Pierre Wehler & Julia Roensch & Roland Eils & Barbara Di Ventura, 2016. "Optogenetic control of nuclear protein export," Nature Communications, Nature, vol. 7(1), pages 1-9, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10624
    DOI: 10.1038/ncomms10624
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    Cited by:

    1. Salim Megat & Natalia Mora & Jason Sanogo & Olga Roman & Alberto Catanese & Najwa Ouali Alami & Axel Freischmidt & Xhuljana Mingaj & Hortense Calbiac & François Muratet & Sylvie Dirrig-Grosch & Stépha, 2023. "Integrative genetic analysis illuminates ALS heritability and identifies risk genes," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Kirstin Meyer & Nicholas C. Lammers & Lukasz J. Bugaj & Hernan G. Garcia & Orion D. Weiner, 2023. "Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Liyuan Zhu & Harold M. McNamara & Jared E. Toettcher, 2023. "Light-switchable transcription factors obtained by direct screening in mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Jiaxi Zhao & Nicholas C. Lammers & Simon Alamos & Yang Joon Kim & Gabriella Martini & Hernan G. Garcia, 2024. "Optogenetic dissection of transcriptional repression in a multicellular organism," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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