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A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans

Author

Listed:
  • Wei Cao

    (Monash University)

  • Qi Fan

    (Monash University)

  • Gemmarie Amparado

    (Monash University)

  • Dean Begic

    (Monash University)

  • Rasoul Godini

    (Monash University)

  • Sandeep Gopal

    (Monash University
    Lund University)

  • Roger Pocock

    (Monash University)

Abstract

Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.

Suggested Citation

  • Wei Cao & Qi Fan & Gemmarie Amparado & Dean Begic & Rasoul Godini & Sandeep Gopal & Roger Pocock, 2024. "A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51212-0
    DOI: 10.1038/s41467-024-51212-0
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