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A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans

Author

Listed:
  • Sarah L. Crittenden

    (University of Wisconsin–Madison)

  • David S. Bernstein

    (University of Wisconsin–Madison)

  • Jennifer L. Bachorik

    (University of Wisconsin–Madison)

  • Beth E. Thompson

    (University of Wisconsin–Madison)

  • Maria Gallegos

    (University of Wisconsin–Madison
    The University of California)

  • Andrei G. Petcherski

    (University of Wisconsin–Madison
    Wormbase)

  • Gary Moulder

    (Oklahoma Medical Research Foundation)

  • Robert Barstead

    (Oklahoma Medical Research Foundation)

  • Marvin Wickens

    (University of Wisconsin–Madison)

  • Judith Kimble

    (University of Wisconsin–Madison
    University of Wisconsin–Madison)

Abstract

Germline stem cells are defined by their unique ability to generate more of themselves as well as differentiated gametes1. The molecular mechanisms controlling the decision between self-renewal and differentiation are central unsolved problems in developmental biology with potentially broad medical implications. In Caenorhabditis elegans, germline stem cells are controlled by the somatic distal tip cell2,3. FBF-1 and FBF-2, two nearly identical proteins, which together are called FBF (‘fem-3 mRNA binding factor’), were originally discovered as regulators of germline sex determination4. Here we report that FBF also controls germline stem cells: in an fbf-1 fbf-2 double mutant, germline proliferation is initially normal, but stem cells are not maintained. We suggest that FBF controls germline stem cells, at least in part, by repressing gld-1, which itself promotes commitment to the meiotic cell cycle5,6. FBF belongs to the PUF family (‘Pumilio and FBF’) of RNA-binding proteins7. Pumilio controls germline stem cells in Drosophila females8,9, and, in lower eukaryotes, PUF proteins promote continued mitoses10,11. We suggest that regulation by PUF proteins may be an ancient and widespread mechanism for control of stem cells.

Suggested Citation

  • Sarah L. Crittenden & David S. Bernstein & Jennifer L. Bachorik & Beth E. Thompson & Maria Gallegos & Andrei G. Petcherski & Gary Moulder & Robert Barstead & Marvin Wickens & Judith Kimble, 2002. "A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans," Nature, Nature, vol. 417(6889), pages 660-663, June.
    • Handle: RePEc:nat:nature:v:417:y:2002:i:6889:d:10.1038_nature754
    DOI: 10.1038/nature754
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    Cited by:

    1. Chen Qiu & Zihan Zhang & Robert N. Wine & Zachary T. Campbell & Jun Zhang & Traci M. Tanaka Hall, 2023. "Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Wei Cao & Qi Fan & Gemmarie Amparado & Dean Begic & Rasoul Godini & Sandeep Gopal & Roger Pocock, 2024. "A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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