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Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry

Author

Listed:
  • Edwin Lasonder

    (University of Southern Denmark
    University of Nijmegen)

  • Yasushi Ishihama

    (University of Southern Denmark)

  • Jens S. Andersen

    (University of Southern Denmark)

  • Adriaan M. W. Vermunt

    (University of Nijmegen)

  • Arnab Pain

    (The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus)

  • Robert W. Sauerwein

    (University Medical Centre)

  • Wijnand M. C. Eling

    (University Medical Centre)

  • Neil Hall

    (The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus)

  • Andrew P. Waters

    (Leiden University Medical Center)

  • Hendrik G. Stunnenberg

    (University of Nijmegen)

  • Matthias Mann

    (University of Southern Denmark)

Abstract

The annotated genomes of organisms define a ‘blueprint’ of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis1,2,3 of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell–cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods.

Suggested Citation

  • Edwin Lasonder & Yasushi Ishihama & Jens S. Andersen & Adriaan M. W. Vermunt & Arnab Pain & Robert W. Sauerwein & Wijnand M. C. Eling & Neil Hall & Andrew P. Waters & Hendrik G. Stunnenberg & Matthias, 2002. "Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry," Nature, Nature, vol. 419(6906), pages 537-542, October.
  • Handle: RePEc:nat:nature:v:419:y:2002:i:6906:d:10.1038_nature01111
    DOI: 10.1038/nature01111
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    Cited by:

    1. Mubasher Mohammed & Alexis Dziedziech & Diego Macedo & Frederik Huppertz & Ylva Veith & Zoé Postel & Elena Christ & Richard Scheytt & Tanja Slotte & Johan Henriksson & Johan Ankarklev, 2024. "Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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