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Neuronal and non-neuronal functions of the synaptic cell adhesion molecule neurexin in Nematostella vectensis

Author

Listed:
  • Christine Guzman

    (Okinawa Institute of Science and Technology Graduate University
    University of Fribourg)

  • Kurato Mohri

    (Okinawa Institute of Science and Technology Graduate University)

  • Ryotaro Nakamura

    (Okinawa Institute of Science and Technology Graduate University)

  • Minato Miyake

    (Okinawa Institute of Science and Technology Graduate University)

  • Yuko Tsuchiya

    (National Institute of Advanced Industrial Science and Technology (AIST))

  • Kentaro Tomii

    (National Institute of Advanced Industrial Science and Technology (AIST))

  • Hiroshi Watanabe

    (Okinawa Institute of Science and Technology Graduate University)

Abstract

The evolutionary transition from diffusion-mediated cell-cell communication to faster, targeted synaptic signaling in animal nervous systems is still unclear. Genome sequencing analyses have revealed a widespread distribution of synapse-related genes among early-diverging metazoans, but how synaptic machinery evolved remains largely unknown. Here, we examine the function of neurexins (Nrxns), a family of presynaptic cell adhesion molecules with critical roles in bilaterian chemical synapses, using the cnidarian model, Nematostella vectensis. Delta-Nrxns are expressed mainly in neuronal cell clusters that exhibit both peptidergic and classical neurotransmitter signaling. Knockdown of δ-Nrxn reduces spontaneous peristalsis of N. vectensis polyps. Interestingly, gene knockdown and pharmacological studies suggest that δ-Nrxn is involved in glutamate- and glycine-mediated signaling rather than peptidergic signaling. Knockdown of the epithelial α-Nrxn reveals a major role in cell adhesion between ectodermal and endodermal epithelia. Overall, this study provides molecular, functional, and cellular insights into the pre-neural function of Nrxns, as well as key information for understanding how and why they were recruited to the synaptic machinery.

Suggested Citation

  • Christine Guzman & Kurato Mohri & Ryotaro Nakamura & Minato Miyake & Yuko Tsuchiya & Kentaro Tomii & Hiroshi Watanabe, 2024. "Neuronal and non-neuronal functions of the synaptic cell adhesion molecule neurexin in Nematostella vectensis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50818-8
    DOI: 10.1038/s41467-024-50818-8
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