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TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis

Author

Listed:
  • Aissam Ikmi

    (Stowers Institute for Medical Research)

  • Sean A. McKinney

    (Stowers Institute for Medical Research)

  • Kym M. Delventhal

    (Stowers Institute for Medical Research)

  • Matthew C. Gibson

    (Stowers Institute for Medical Research
    University of Kansas School of Medicine)

Abstract

Non-bilaterian phyla represent key lineages for exploring the evolutionary history of early animals. However, despite an increasing number of sequenced genomes from early-branching metazoans, efficient and reproducible methodologies for analysis of gene function remain a major challenge. Here we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutations and homologous recombination-mediated transgenesis in the sea anemone Nematostella vectensis. We also present a new method to isolate genetically modified animals using engineered selection cassettes introduced by homologous recombination. Taken together, these methods will permit sophisticated gain- and loss-of-function analyses in Nematostella and perhaps other early metazoan species that allow for zygotic injection.

Suggested Citation

  • Aissam Ikmi & Sean A. McKinney & Kym M. Delventhal & Matthew C. Gibson, 2014. "TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6486
    DOI: 10.1038/ncomms6486
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    Cited by:

    1. Christine Guzman & Kurato Mohri & Ryotaro Nakamura & Minato Miyake & Yuko Tsuchiya & Kentaro Tomii & Hiroshi Watanabe, 2024. "Neuronal and non-neuronal functions of the synaptic cell adhesion molecule neurexin in Nematostella vectensis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Leslie S. Babonis & Camille Enjolras & Abigail J. Reft & Brent M. Foster & Fredrik Hugosson & Joseph F. Ryan & Marymegan Daly & Mark Q. Martindale, 2023. "Single-cell atavism reveals an ancient mechanism of cell type diversification in a sea anemone," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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