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The Escherichia coli chromosome moves to the replisome

Author

Listed:
  • Konrad Gras

    (Uppsala University)

  • David Fange

    (Uppsala University)

  • Johan Elf

    (Uppsala University)

Abstract

In Escherichia coli, it is debated whether the two replisomes move independently along the two chromosome arms during replication or if they remain spatially confined. Here, we use high-throughput fluorescence microscopy to simultaneously determine the location and short-time-scale (1 s) movement of the replisome and a chromosomal locus throughout the cell cycle. The assay is performed for several loci. We find that (i) the two replisomes are confined to a region of ~250 nm and ~120 nm along the cell’s long and short axis, respectively, (ii) the chromosomal loci move to and through this region sequentially based on their distance from the origin of replication, and (iii) when a locus is being replicated, its short time-scale movement slows down. This behavior is the same at different growth rates. In conclusion, our data supports a model with DNA moving towards spatially confined replisomes at replication.

Suggested Citation

  • Konrad Gras & David Fange & Johan Elf, 2024. "The Escherichia coli chromosome moves to the replisome," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50047-z
    DOI: 10.1038/s41467-024-50047-z
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    References listed on IDEAS

    as
    1. Sarah M Mangiameli & Brian T Veit & Houra Merrikh & Paul A Wiggins, 2017. "The Replisomes Remain Spatially Proximal throughout the Cell Cycle in Bacteria," PLOS Genetics, Public Library of Science, vol. 13(1), pages 1-17, January.
    2. M. Charl Moolman & Sriram Tiruvadi Krishnan & Jacob W. J. Kerssemakers & Aafke van den Berg & Pawel Tulinski & Martin Depken & Rodrigo Reyes-Lamothe & David J. Sherratt & Nynke H. Dekker, 2014. "Slow unloading leads to DNA-bound β2-sliding clamp accumulation in live Escherichia coli cells," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    3. Martin Lindén & Vladimir Ćurić & Elias Amselem & Johan Elf, 2017. "Pointwise error estimates in localization microscopy," Nature Communications, Nature, vol. 8(1), pages 1-9, August.
    4. Aleksandre Japaridze & Christos Gogou & Jacob W. J. Kerssemakers & Huyen My Nguyen & Cees Dekker, 2020. "Direct observation of independently moving replisomes in Escherichia coli," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    5. Avelino Javer & Zhicheng Long & Eileen Nugent & Marco Grisi & Kamin Siriwatwetchakul & Kevin D. Dorfman & Pietro Cicuta & Marco Cosentino Lagomarsino, 2013. "Short-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization," Nature Communications, Nature, vol. 4(1), pages 1-8, October.
    6. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
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