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MatP local enrichment delays segregation independently of tetramer formation and septal anchoring in Vibrio cholerae

Author

Listed:
  • Elena Espinosa

    (1 Avenue de la Terrasse)

  • Jihane Challita

    (1 Avenue de la Terrasse)

  • Jean-Michel Desfontaines

    (1 Avenue de la Terrasse)

  • Christophe Possoz

    (1 Avenue de la Terrasse)

  • Marie-Eve Val

    (Unité Plasticité du Génome Bactérien)

  • Didier Mazel

    (Unité Plasticité du Génome Bactérien)

  • Martial Marbouty

    (Unité Spatial Regulation of Genomes)

  • Romain Koszul

    (Unité Spatial Regulation of Genomes)

  • Elisa Galli

    (1 Avenue de la Terrasse)

  • François-Xavier Barre

    (1 Avenue de la Terrasse)

Abstract

Vibrio cholerae harbours a primary chromosome derived from the monochromosomal ancestor of the Vibrionales (ChrI) and a secondary chromosome derived from a megaplasmid (ChrII). The coordinated segregation of the replication terminus of both chromosomes (TerI and TerII) determines when and where cell division occurs. ChrI encodes a homologue of Escherichia coli MatP, a protein that binds to a DNA motif (matS) that is overrepresented in replication termini. Here, we use a combination of deep sequencing and fluorescence microscopy techniques to show that V. cholerae MatP structures TerI and TerII into macrodomains, targets them to mid-cell during replication, and delays their segregation, thus supporting that ChrII behaves as a bona fide chromosome. We further show that the extent of the segregation delay mediated by MatP depends on the number and local density of matS sites, and is independent of its assembly into tetramers and any interaction with the divisome, in contrast to what has been previously observed in E. coli.

Suggested Citation

  • Elena Espinosa & Jihane Challita & Jean-Michel Desfontaines & Christophe Possoz & Marie-Eve Val & Didier Mazel & Martial Marbouty & Romain Koszul & Elisa Galli & François-Xavier Barre, 2024. "MatP local enrichment delays segregation independently of tetramer formation and septal anchoring in Vibrio cholerae," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54195-0
    DOI: 10.1038/s41467-024-54195-0
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    References listed on IDEAS

    as
    1. Estelle Crozat & Catherine Tardin & Maya Salhi & Philippe Rousseau & Armand Lablaine & Tommaso Bertoni & David Holcman & Bianca Sclavi & Pietro Cicuta & François Cornet, 2020. "Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    2. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
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    1. Ismath Sadhir & Seán M. Murray, 2023. "Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Konrad Gras & David Fange & Johan Elf, 2024. "The Escherichia coli chromosome moves to the replisome," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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