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Short-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization

Author

Listed:
  • Avelino Javer

    (Cavendish Laboratory, University of Cambridge)

  • Zhicheng Long

    (University of Minnesota—Twin Cities)

  • Eileen Nugent

    (Cavendish Laboratory, University of Cambridge)

  • Marco Grisi

    (Cavendish Laboratory, University of Cambridge
    University of Milano)

  • Kamin Siriwatwetchakul

    (Cavendish Laboratory, University of Cambridge)

  • Kevin D. Dorfman

    (University of Minnesota—Twin Cities)

  • Pietro Cicuta

    (Cavendish Laboratory, University of Cambridge)

  • Marco Cosentino Lagomarsino

    (Genomic Physics Group, UMR 7238 CNRS ‘Microorganism Genomics’, 15, rue de l’École de M´edecine
    University Pierre et Marie Curie, 15, rue de l’École de M´edecine
    University of Torino)

Abstract

In bacteria, chromosomal architecture shows strong spatial and temporal organization, and regulates key cellular functions, such as transcription. Tracking the motion of chromosomal loci at short timescales provides information related to both the physical state of the nucleo–protein complex and its local environment, independent of large-scale motions related to genome segregation. Here we investigate the short-time (0.1–10 s) dynamics of fluorescently labelled chromosomal loci in Escherichia coli at different growth rates. At these timescales, we observe for the first time a dependence of the loci’s apparent diffusion on both their subcellular localization and chromosomal coordinate, and we provide evidence that the properties of the chromosome are similar in the tested growth conditions. Our results indicate that either non-equilibrium fluctuations due to enzyme activity or the organization of the genome as a polymer–protein complex vary as a function of the distance from the origin of replication.

Suggested Citation

  • Avelino Javer & Zhicheng Long & Eileen Nugent & Marco Grisi & Kamin Siriwatwetchakul & Kevin D. Dorfman & Pietro Cicuta & Marco Cosentino Lagomarsino, 2013. "Short-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization," Nature Communications, Nature, vol. 4(1), pages 1-8, October.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3003
    DOI: 10.1038/ncomms3003
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    Cited by:

    1. Konrad Gras & David Fange & Johan Elf, 2024. "The Escherichia coli chromosome moves to the replisome," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Ofir Shukron & David Holcman, 2017. "Transient chromatin properties revealed by polymer models and stochastic simulations constructed from Chromosomal Capture data," PLOS Computational Biology, Public Library of Science, vol. 13(4), pages 1-20, April.

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