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Direct observation of independently moving replisomes in Escherichia coli

Author

Listed:
  • Aleksandre Japaridze

    (Delft University of Technology)

  • Christos Gogou

    (Delft University of Technology)

  • Jacob W. J. Kerssemakers

    (Delft University of Technology)

  • Huyen My Nguyen

    (Delft University of Technology)

  • Cees Dekker

    (Delft University of Technology)

Abstract

The replication and transfer of genomic material from a cell to its progeny are vital processes in all living systems. Here we visualize the process of chromosome replication in widened E. coli cells. Monitoring the replication of single chromosomes yields clear examples of replication bubbles that reveal that the two replisomes move independently from the origin to the terminus of replication along each of the two arms of the circular chromosome, providing direct support for the so-called train-track model, and against a factory model for replisomes. The origin of replication duplicates near midcell, initially splitting to random directions and subsequently towards the poles. The probability of successful segregation of chromosomes significantly decreases with increasing cell width, indicating that chromosome confinement by the cell boundary is an important driver of DNA segregation. Our findings resolve long standing questions in bacterial chromosome organization.

Suggested Citation

  • Aleksandre Japaridze & Christos Gogou & Jacob W. J. Kerssemakers & Huyen My Nguyen & Cees Dekker, 2020. "Direct observation of independently moving replisomes in Escherichia coli," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16946-7
    DOI: 10.1038/s41467-020-16946-7
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    Cited by:

    1. Konrad Gras & David Fange & Johan Elf, 2024. "The Escherichia coli chromosome moves to the replisome," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Miloš Tišma & Florian Patrick Bock & Jacob Kerssemakers & Hammam Antar & Aleksandre Japaridze & Stephan Gruber & Cees Dekker, 2024. "Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Chen Zhang & Asha Mary Joseph & Laurent Casini & Justine Collier & Anjana Badrinarayanan & Suliana Manley, 2024. "Chromosome organization shapes replisome dynamics in Caulobacter crescentus," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    4. David Geisel & Peter Lenz, 2022. "Machine learning classification of trajectories from molecular dynamics simulations of chromosome segregation," PLOS ONE, Public Library of Science, vol. 17(1), pages 1-33, January.

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