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Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin

Author

Listed:
  • Sayaka Matsui

    (Nagoya University)

  • Saki Noda

    (Nagoya University)

  • Keiko Kuwata

    (Nagoya University)

  • Mika Nomoto

    (Nagoya University)

  • Yasuomi Tada

    (Nagoya University)

  • Hidefumi Shinohara

    (Nagoya University
    Fukui Prefectural University)

  • Yoshikatsu Matsubayashi

    (Nagoya University)

Abstract

Plants initiate specific defense responses by recognizing conserved epitope peptides within the flagellin proteins derived from bacteria. Proteolytic cleavage of epitope peptides from flagellin by plant apoplastic proteases is thought to be crucial for the perception of the epitope by the plant receptor. However, the identity of the plant proteases involved in this process remains unknown. Here, we establish an efficient identification system for the target proteases in Arabidopsis apoplastic fluid; the method employs native two-dimensional electrophoresis followed by an in-gel proteolytic assay using a fluorescence-quenching peptide substrate. We designed a substrate to specifically detect proteolytic activity at the C-terminus of the flg22 epitope in flagellin and identified two plant subtilases, SBT5.2 and SBT1.7, as specific proteases responsible for the C-terminal cleavage of flg22. In the apoplastic fluid of Arabidopsis mutant plants deficient in these two proteases, we observe a decrease in the C-terminal cleavage of the flg22 domain from flagellin, leading to a decrease in the efficiency of flg22 epitope liberation. Consequently, defensive reactive oxygen species (ROS) production is delayed in sbt5.2 sbt1.7 double-mutant leaf disks compared to wild type following flagellin exposure.

Suggested Citation

  • Sayaka Matsui & Saki Noda & Keiko Kuwata & Mika Nomoto & Yasuomi Tada & Hidefumi Shinohara & Yoshikatsu Matsubayashi, 2024. "Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48108-4
    DOI: 10.1038/s41467-024-48108-4
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    References listed on IDEAS

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    1. Cawas B. Engineer & Majid Ghassemian & Jeffrey C. Anderson & Scott C. Peck & Honghong Hu & Julian I. Schroeder, 2014. "Carbonic anhydrases, EPF2 and a novel protease mediate CO2 control of stomatal development," Nature, Nature, vol. 513(7517), pages 246-250, September.
    2. Bruno Pok Man Ngou & Hee-Kyung Ahn & Pingtao Ding & Jonathan D. G. Jones, 2021. "Mutual potentiation of plant immunity by cell-surface and intracellular receptors," Nature, Nature, vol. 592(7852), pages 110-115, April.
    3. Cyril Zipfel & Silke Robatzek & Lionel Navarro & Edward J. Oakeley & Jonathan D. G. Jones & Georg Felix & Thomas Boller, 2004. "Bacterial disease resistance in Arabidopsis through flagellin perception," Nature, Nature, vol. 428(6984), pages 764-767, April.
    4. Minhang Yuan & Zeyu Jiang & Guozhi Bi & Kinya Nomura & Menghui Liu & Yiping Wang & Boying Cai & Jian-Min Zhou & Sheng Yang He & Xiu-Fang Xin, 2021. "Pattern-recognition receptors are required for NLR-mediated plant immunity," Nature, Nature, vol. 592(7852), pages 105-109, April.
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