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ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA

Author

Listed:
  • Xinlei Wei

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Xue Yang

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Congcong Hu

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology)

  • Qiangzi Li

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District)

  • Qianqian Liu

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Yue Wu

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Leipeng Xie

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Xiao Ning

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District)

  • Fei Li

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Tao Cai

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Zhiguang Zhu

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District
    National Technology Innovation Center of Synthetic Biology)

  • Yi-Heng P. Job Zhang

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District
    National Technology Innovation Center of Synthetic Biology)

  • Yanfei Zhang

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District
    National Technology Innovation Center of Synthetic Biology)

  • Xuejun Chen

    (Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area)

  • Chun You

    (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area
    Shijingshan District
    National Technology Innovation Center of Synthetic Biology)

Abstract

In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP+/NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch.

Suggested Citation

  • Xinlei Wei & Xue Yang & Congcong Hu & Qiangzi Li & Qianqian Liu & Yue Wu & Leipeng Xie & Xiao Ning & Fei Li & Tao Cai & Zhiguang Zhu & Yi-Heng P. Job Zhang & Yanfei Zhang & Xuejun Chen & Chun You, 2024. "ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46871-y
    DOI: 10.1038/s41467-024-46871-y
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    References listed on IDEAS

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