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A synthetic biochemistry molecular purge valve module that maintains redox balance

Author

Listed:
  • Paul H. Opgenorth

    (UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California)

  • Tyler P. Korman

    (UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California)

  • James U. Bowie

    (UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California
    Boyer Hall, UCLA)

Abstract

The greatest potential environmental benefit of metabolic engineering would be the production of high-volume commodity chemicals, such as biofuels. Yet, the high yields required for the economic viability of low-value chemicals is particularly hard to achieve in microbes owing to the myriad competing biochemical pathways. An alternative approach, which we call synthetic biochemistry, is to eliminate the organism by constructing biochemical pathways in vitro. Viable synthetic biochemistry, however, will require simple methods to replace the cellular circuitry that maintains cofactor balance. Here we design a simple purge valve module for maintaining NADP+/NADPH balance. We test the purge valve in the production of polyhydroxybutyryl bioplastic and isoprene—pathways where cofactor generation and utilization are unbalanced. We find that the regulatory system is highly robust to variations in cofactor levels and readily transportable. The molecular purge valve provides a step towards developing continuously operating, sustainable synthetic biochemistry systems.

Suggested Citation

  • Paul H. Opgenorth & Tyler P. Korman & James U. Bowie, 2014. "A synthetic biochemistry molecular purge valve module that maintains redox balance," Nature Communications, Nature, vol. 5(1), pages 1-8, September.
  • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5113
    DOI: 10.1038/ncomms5113
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    Cited by:

    1. Xinlei Wei & Xue Yang & Congcong Hu & Qiangzi Li & Qianqian Liu & Yue Wu & Leipeng Xie & Xiao Ning & Fei Li & Tao Cai & Zhiguang Zhu & Yi-Heng P. Job Zhang & Yanfei Zhang & Xuejun Chen & Chun You, 2024. "ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Chen, Hong-Ge & Zhang, Y.-H. Percival, 2015. "New biorefineries and sustainable agriculture: Increased food, biofuels, and ecosystem security," Renewable and Sustainable Energy Reviews, Elsevier, vol. 47(C), pages 117-132.

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