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Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria

Author

Listed:
  • Ryan Puskar

    (Arizona State University
    Arizona State University)

  • Chloe Truong

    (Arizona State University
    Arizona State University
    Rampart Bioscience)

  • Kyle Swain

    (Arizona State University)

  • Saborni Chowdhury

    (Arizona State University
    Arizona State University)

  • Ka-Yi Chan

    (Arizona State University
    Arizona State University)

  • Shan Li

    (California Institute of Technology)

  • Kai-Wen Cheng

    (California Institute of Technology)

  • Ting Yu Wang

    (California Institute of Technology)

  • Yu-Ping Poh

    (Arizona State University
    Arizona State University)

  • Yuval Mazor

    (Arizona State University
    Arizona State University)

  • Haijun Liu

    (Washington University)

  • Tsui-Fen Chou

    (California Institute of Technology
    California Institute of Technology)

  • Brent L. Nannenga

    (Arizona State University
    Arizona State University)

  • Po-Lin Chiu

    (Arizona State University
    Arizona State University)

Abstract

The photochemical reaction center (RC) features a dimeric architecture for charge separation across the membrane. In green sulfur bacteria (GSB), the trimeric Fenna-Matthews-Olson (FMO) complex mediates the transfer of light energy from the chlorosome antenna complex to the RC. Here we determine the structure of the photosynthetic supercomplex from the GSB Chlorobaculum tepidum using single-particle cryogenic electron microscopy (cryo-EM) and identify the cytochrome c subunit (PscC), two accessory protein subunits (PscE and PscF), a second FMO trimeric complex, and a linker pigment between FMO and the RC core. The protein subunits that are assembled with the symmetric RC core generate an asymmetric photosynthetic supercomplex. One linker bacteriochlorophyll (BChl) is located in one of the two FMO-PscA interfaces, leading to differential efficiencies of the two energy transfer branches. The two FMO trimeric complexes establish two different binding interfaces with the RC cytoplasmic surface, driven by the associated accessory subunits. This structure of the GSB photosynthetic supercomplex provides mechanistic insight into the light excitation energy transfer routes and a possible evolutionary transition intermediate of the bacterial photosynthetic supercomplex from the primitive homodimeric RC.

Suggested Citation

  • Ryan Puskar & Chloe Truong & Kyle Swain & Saborni Chowdhury & Ka-Yi Chan & Shan Li & Kai-Wen Cheng & Ting Yu Wang & Yu-Ping Poh & Yuval Mazor & Haijun Liu & Tsui-Fen Chou & Brent L. Nannenga & Po-Lin , 2022. "Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33505-4
    DOI: 10.1038/s41467-022-33505-4
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    References listed on IDEAS

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    Cited by:

    1. Shishang Dong & Guoqiang Huang & Changhui Wang & Jiajia Wang & Sen-Fang Sui & Xiaochun Qin, 2022. "Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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