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Dual-wavelength metalens enables Epi-fluorescence detection from single molecules

Author

Listed:
  • Aleksandr Barulin

    (Sungkyunkwan University
    Sungkyunkwan University)

  • Yeseul Kim

    (Pohang University of Science and Technology (POSTECH))

  • Dong Kyo Oh

    (Pohang University of Science and Technology (POSTECH))

  • Jaehyuck Jang

    (Pohang University of Science and Technology (POSTECH)
    POSCO-POSTECH-RIST Convergence Research Center for Flat Optics and Metaphotonics)

  • Hyemi Park

    (Sungkyunkwan University
    Sungkyunkwan University)

  • Junsuk Rho

    (Pohang University of Science and Technology (POSTECH)
    Pohang University of Science and Technology (POSTECH)
    POSCO-POSTECH-RIST Convergence Research Center for Flat Optics and Metaphotonics
    National Institute of Nanomaterials Technology (NINT))

  • Inki Kim

    (Sungkyunkwan University
    Sungkyunkwan University)

Abstract

Single molecule fluorescence spectroscopy is at the heart of molecular biophysics research and the most sensitive biosensing assays. The growing demand for precision medicine and environmental monitoring requires the creation of miniaturized and portable sensing platforms. However, the need for highly sophisticated objective lenses has precluded the development of single molecule detection systems for truly portable devices. Here, we propose a dielectric metalens device of submicrometer thickness to excite and collect light from fluorescent molecules instead of an objective lens. The high numerical aperture, high focusing efficiency, and dual-wavelength operation of the metalens enable the implementation of fluorescence correlation spectroscopy with a single Alexa 647 molecule in the focal volume. Moreover, the metalens enables real-time monitoring of individual fluorescent nanoparticle transitions and identification of hydrodynamic diameters ranging from a few to hundreds of nanometers. This advancement in sensitivity extends the application of the metalens technology to ultracompact single-molecule sensors.

Suggested Citation

  • Aleksandr Barulin & Yeseul Kim & Dong Kyo Oh & Jaehyuck Jang & Hyemi Park & Junsuk Rho & Inki Kim, 2024. "Dual-wavelength metalens enables Epi-fluorescence detection from single molecules," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44407-4
    DOI: 10.1038/s41467-023-44407-4
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    References listed on IDEAS

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    1. Kateryna Trofymchuk & Viktorija Glembockyte & Lennart Grabenhorst & Florian Steiner & Carolin Vietz & Cindy Close & Martina Pfeiffer & Lars Richter & Max L. Schütte & Florian Selbach & Renukka Yaadav , 2021. "Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Tzu-Yung Huang & Richard R. Grote & Sander A. Mann & David A. Hopper & Annemarie L. Exarhos & Gerald G. Lopez & Amelia R. Klein & Erik C. Garnett & Lee C. Bassett, 2019. "A monolithic immersion metalens for imaging solid-state quantum emitters," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    3. Haoran Ren & Jaehyuck Jang & Chenhao Li & Andreas Aigner & Malte Plidschun & Jisoo Kim & Junsuk Rho & Markus A. Schmidt & Stefan A. Maier, 2022. "An achromatic metafiber for focusing and imaging across the entire telecommunication range," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    4. Aleksandr Barulin & Prithu Roy & Jean-Benoît Claude & Jérôme Wenger, 2022. "Ultraviolet optical horn antennas for label-free detection of single proteins," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    5. F. Balli & M. Sultan & Sarah K. Lami & J. T. Hastings, 2020. "A hybrid achromatic metalens," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
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