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Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

Author

Listed:
  • Kateryna Trofymchuk

    (Ludwig-Maximilians-Universität München
    Technische Universität Braunschweig)

  • Viktorija Glembockyte

    (Ludwig-Maximilians-Universität München)

  • Lennart Grabenhorst

    (Ludwig-Maximilians-Universität München)

  • Florian Steiner

    (Ludwig-Maximilians-Universität München)

  • Carolin Vietz

    (Technische Universität Braunschweig)

  • Cindy Close

    (Ludwig-Maximilians-Universität München)

  • Martina Pfeiffer

    (Ludwig-Maximilians-Universität München)

  • Lars Richter

    (Technische Universität Braunschweig)

  • Max L. Schütte

    (Technische Universität Braunschweig)

  • Florian Selbach

    (Ludwig-Maximilians-Universität München)

  • Renukka Yaadav

    (Ludwig-Maximilians-Universität München)

  • Jonas Zähringer

    (Ludwig-Maximilians-Universität München)

  • Qingshan Wei

    (North Carolina State University)

  • Aydogan Ozcan

    (University of California)

  • Birka Lalkens

    (Laboratory for Emerging Nanometrology LENA)

  • Guillermo P. Acuna

    (Université de Fribourg - Faculté des Sciences et Médicine)

  • Philip Tinnefeld

    (Ludwig-Maximilians-Universität München)

Abstract

The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.

Suggested Citation

  • Kateryna Trofymchuk & Viktorija Glembockyte & Lennart Grabenhorst & Florian Steiner & Carolin Vietz & Cindy Close & Martina Pfeiffer & Lars Richter & Max L. Schütte & Florian Selbach & Renukka Yaadav , 2021. "Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21238-9
    DOI: 10.1038/s41467-021-21238-9
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    Cited by:

    1. Aleksandr Barulin & Yeseul Kim & Dong Kyo Oh & Jaehyuck Jang & Hyemi Park & Junsuk Rho & Inki Kim, 2024. "Dual-wavelength metalens enables Epi-fluorescence detection from single molecules," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Amani A. Hariri & Sharon S. Newman & Steven Tan & Dan Mamerow & Alexandra M. Adams & Nicolò Maganzini & Brian L. Zhong & Michael Eisenstein & Alexander R. Dunn & H. Tom Soh, 2022. "Improved immunoassay sensitivity and specificity using single-molecule colocalization," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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