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GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis

Author

Listed:
  • Qiang Zhu

    (University of North Carolina)

  • Matthew E. Combs

    (University of North Carolina)

  • Juan Liu

    (Duke University School of Medicine)

  • Xue Bai

    (University of North Carolina)

  • Wenbo B. Wang

    (University of North Carolina)

  • Laura E. Herring

    (University of North Carolina)

  • Jiandong Liu

    (University of North Carolina
    McAllister Heart Institute University of North Carolina)

  • Jason W. Locasale

    (Duke University School of Medicine)

  • Dawn E. Bowles

    (Duke University Medical Center)

  • Ryan T. Gross

    (Duke University Medical Center)

  • Michelle Mendiola Pla

    (Duke University Medical Center)

  • Christopher P. Mack

    (University of North Carolina
    McAllister Heart Institute University of North Carolina)

  • Joan M. Taylor

    (University of North Carolina
    McAllister Heart Institute University of North Carolina)

Abstract

The serine/threonine kinase, PINK1, and the E3 ubiquitin ligase, Parkin, are known to facilitate LC3-dependent autophagosomal encasement and lysosomal clearance of dysfunctional mitochondria, and defects in this process contribute to a variety of cardiometabolic and neurological diseases. Although recent evidence indicates that dynamic actin remodeling plays an important role in PINK1/Parkin-mediated mitochondrial autophagy (mitophagy), the underlying signaling mechanisms remain unknown. Here, we identify the RhoGAP GRAF1 (Arhgap26) as a PINK1 substrate that regulates mitophagy. GRAF1 promotes the release of damaged mitochondria from F-actin anchors, regulates mitochondrial-associated Arp2/3-mediated actin remodeling and facilitates Parkin-LC3 interactions to enhance mitochondria capture by autophagosomes. Graf1 phosphorylation on PINK1-dependent sites is dysregulated in human heart failure, and cardiomyocyte-restricted Graf1 depletion in mice blunts mitochondrial clearance and attenuates compensatory metabolic adaptations to stress. Overall, we identify GRAF1 as an enzyme that coordinates cytoskeletal and metabolic remodeling to promote cardioprotection.

Suggested Citation

  • Qiang Zhu & Matthew E. Combs & Juan Liu & Xue Bai & Wenbo B. Wang & Laura E. Herring & Jiandong Liu & Jason W. Locasale & Dawn E. Bowles & Ryan T. Gross & Michelle Mendiola Pla & Christopher P. Mack &, 2023. "GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis," Nature Communications, Nature, vol. 14(1), pages 1-21, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43889-6
    DOI: 10.1038/s41467-023-43889-6
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    References listed on IDEAS

    as
    1. Cheng-Wei Hsieh & Wei Yuan Yang, 2019. "Omegasome-proximal PtdIns(4,5)P2 couples F-actin mediated mitoaggregate disassembly with autophagosome formation during mitophagy," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    2. Christina Gladkova & Sarah L. Maslen & J. Mark Skehel & David Komander, 2018. "Mechanism of parkin activation by PINK1," Nature, Nature, vol. 559(7714), pages 410-414, July.
    3. Shireen A. Sarraf & Malavika Raman & Virginia Guarani-Pereira & Mathew E. Sowa & Edward L. Huttlin & Steven P. Gygi & J. Wade Harper, 2013. "Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization," Nature, Nature, vol. 496(7445), pages 372-376, April.
    4. Michael Lazarou & Danielle A. Sliter & Lesley A. Kane & Shireen A. Sarraf & Chunxin Wang & Jonathon L. Burman & Dionisia P. Sideris & Adam I. Fogel & Richard J. Youle, 2015. "The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy," Nature, Nature, vol. 524(7565), pages 309-314, August.
    5. Ilija Melentijevic & Marton L. Toth & Meghan L. Arnold & Ryan J. Guasp & Girish Harinath & Ken C. Nguyen & Daniel Taub & J. Alex Parker & Christian Neri & Christopher V. Gabel & David H. Hall & Monica, 2017. "C. elegans neurons jettison protein aggregates and mitochondria under neurotoxic stress," Nature, Nature, vol. 542(7641), pages 367-371, February.
    6. Fumika Koyano & Kei Okatsu & Hidetaka Kosako & Yasushi Tamura & Etsu Go & Mayumi Kimura & Yoko Kimura & Hikaru Tsuchiya & Hidehito Yoshihara & Takatsugu Hirokawa & Toshiya Endo & Edward A. Fon & Jean-, 2014. "Ubiquitin is phosphorylated by PINK1 to activate parkin," Nature, Nature, vol. 510(7503), pages 162-166, June.
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