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Intermediate filaments associate with aggresome-like structures in proteostressed C. elegans neurons and influence large vesicle extrusions as exophers

Author

Listed:
  • Meghan Lee Arnold

    (Rutgers University)

  • Jason Cooper

    (Rutgers University)

  • Rebecca Androwski

    (Rutgers University)

  • Sohil Ardeshna

    (Rutgers University)

  • Ilija Melentijevic

    (Rutgers University)

  • Joelle Smart

    (Rutgers University)

  • Ryan J. Guasp

    (Rutgers University)

  • Ken C. Q. Nguyen

    (Rose F. Kennedy Center)

  • Ge Bai

    (Rutgers University)

  • David H. Hall

    (Rose F. Kennedy Center)

  • Barth D. Grant

    (Rutgers University)

  • Monica Driscoll

    (Rutgers University)

Abstract

Toxic protein aggregates can spread among neurons to promote human neurodegenerative disease pathology. We found that in C. elegans touch neurons intermediate filament proteins IFD-1 and IFD-2 associate with aggresome-like organelles and are required cell-autonomously for efficient production of neuronal exophers, giant vesicles that can carry aggregates away from the neuron of origin. The C. elegans aggresome-like organelles we identified are juxtanuclear, HttPolyQ aggregate-enriched, and dependent upon orthologs of mammalian aggresome adaptor proteins, dynein motors, and microtubule integrity for localized aggregate collection. These key hallmarks indicate that conserved mechanisms drive aggresome formation. Furthermore, we found that human neurofilament light chain (NFL) can substitute for C. elegans IFD-2 in promoting exopher extrusion. Taken together, our results suggest a conserved influence of intermediate filament association with aggresomes and neuronal extrusions that eject potentially toxic material. Our findings expand understanding of neuronal proteostasis and suggest implications for neurodegenerative disease progression.

Suggested Citation

  • Meghan Lee Arnold & Jason Cooper & Rebecca Androwski & Sohil Ardeshna & Ilija Melentijevic & Joelle Smart & Ryan J. Guasp & Ken C. Q. Nguyen & Ge Bai & David H. Hall & Barth D. Grant & Monica Driscoll, 2023. "Intermediate filaments associate with aggresome-like structures in proteostressed C. elegans neurons and influence large vesicle extrusions as exophers," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39700-1
    DOI: 10.1038/s41467-023-39700-1
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    References listed on IDEAS

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    1. Nicholas J. Ashton & Shorena Janelidze & Ahmad Al Khleifat & Antoine Leuzy & Emma L. van der Ende & Thomas K. Karikari & Andrea L. Benedet & Tharick A. Pascoal & Alberto Lleó & Lucilla Parnetti & Dani, 2021. "A multicentre validation study of the diagnostic value of plasma neurofilament light," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Ilija Melentijevic & Marton L. Toth & Meghan L. Arnold & Ryan J. Guasp & Girish Harinath & Ken C. Nguyen & Daniel Taub & J. Alex Parker & Christian Neri & Christopher V. Gabel & David H. Hall & Monica, 2017. "C. elegans neurons jettison protein aggregates and mitochondria under neurotoxic stress," Nature, Nature, vol. 542(7641), pages 367-371, February.
    3. Mohammed D. Aljohani & Sonia El Mouridi & Monika Priyadarshini & Amhed M. Vargas-Velazquez & Christian Frøkjær-Jensen, 2020. "Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
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