IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-43610-7.html
   My bibliography  Save this article

The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2 in the yeast Komagataella phaffii

Author

Listed:
  • Bernd M. Mitic

    (University of Natural Resources and Life Sciences, Vienna, Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18
    University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Institute of Analytical Chemistry, Muthgasse 18)

  • Christina Troyer

    (University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Institute of Analytical Chemistry, Muthgasse 18)

  • Lisa Lutz

    (University of Natural Resources and Life Sciences, Vienna, Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18
    Austrian Centre of Industrial Biotechnology (ACIB), Muthgasse 11)

  • Michael Baumschabl

    (University of Natural Resources and Life Sciences, Vienna, Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18
    Austrian Centre of Industrial Biotechnology (ACIB), Muthgasse 11)

  • Stephan Hann

    (University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Institute of Analytical Chemistry, Muthgasse 18
    Austrian Centre of Industrial Biotechnology (ACIB), Muthgasse 11)

  • Diethard Mattanovich

    (University of Natural Resources and Life Sciences, Vienna, Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, Muthgasse 18
    Austrian Centre of Industrial Biotechnology (ACIB), Muthgasse 11)

Abstract

The current climatic change is predominantly driven by excessive anthropogenic CO2 emissions. As industrial bioprocesses primarily depend on food-competing organic feedstocks or fossil raw materials, CO2 co-assimilation or the use of CO2-derived methanol or formate as carbon sources are considered pathbreaking contributions to solving this global problem. The number of industrially-relevant microorganisms that can use these two carbon sources is limited, and even fewer can concurrently co-assimilate CO2. Here, we search for alternative native methanol and formate assimilation pathways that co-assimilate CO2 in the industrially-relevant methylotrophic yeast Komagataella phaffii (Pichia pastoris). Using 13C-tracer-based metabolomic techniques and metabolic engineering approaches, we discover and confirm a growth supporting pathway based on native enzymes that can perform all three assimilations: namely, the oxygen-tolerant reductive glycine pathway. This finding paves the way towards metabolic engineering of formate and CO2 utilisation to produce proteins, biomass, or chemicals in yeast.

Suggested Citation

  • Bernd M. Mitic & Christina Troyer & Lisa Lutz & Michael Baumschabl & Stephan Hann & Diethard Mattanovich, 2023. "The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2 in the yeast Komagataella phaffii," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43610-7
    DOI: 10.1038/s41467-023-43610-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-43610-7
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-43610-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Monica I. Espinosa & Ricardo A. Gonzalez-Garcia & Kaspar Valgepea & Manuel R. Plan & Colin Scott & Isak S. Pretorius & Esteban Marcellin & Ian T. Paulsen & Thomas C. Williams, 2020. "Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    2. Philipp Keller & Michael A. Reiter & Patrick Kiefer & Thomas Gassler & Lucas Hemmerle & Philipp Christen & Elad Noor & Julia A. Vorholt, 2022. "Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yeonhwa Yu & Yongfan Shi & Young Wan Kwon & Yoobin Choi & Yusik Kim & Jeong-Geol Na & June Huh & Jeewon Lee, 2024. "A rationally designed miniature of soluble methane monooxygenase enables rapid and high-yield methanol production in Escherichia coli," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Enrico Orsi & Pablo Ivan Nikel & Lars Keld Nielsen & Stefano Donati, 2023. "Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Simone Bachleitner & Özge Ata & Diethard Mattanovich, 2023. "The potential of CO2-based production cycles in biotechnology to fight the climate crisis," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    4. Briardo Llorente & Thomas C. Williams & Hugh D. Goold & Isak S. Pretorius & Ian T. Paulsen, 2022. "Harnessing bioengineered microbes as a versatile platform for space nutrition," Nature Communications, Nature, vol. 13(1), pages 1-7, December.
    5. Maren Nattermann & Sebastian Wenk & Pascal Pfister & Hai He & Seung Hwan Lee & Witold Szymanski & Nils Guntermann & Fayin Zhu & Lennart Nickel & Charlotte Wallner & Jan Zarzycki & Nicole Paczia & Nina, 2023. "Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    6. Cong Zhang & Di-Fei Zhou & Meng-Ying Wang & Ya-Zhen Song & Chong Zhang & Ming-Ming Zhang & Jing Sun & Lu Yao & Xu-Hua Mo & Zeng-Xin Ma & Xiao-Jie Yuan & Yi Shao & Hao-Ran Wang & Si-Han Dong & Kai Bao , 2024. "Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    7. Tong Wu & Paul A. Gómez-Coronado & Armin Kubis & Steffen N. Lindner & Philippe Marlière & Tobias J. Erb & Arren Bar-Even & Hai He, 2023. "Engineering a synthetic energy-efficient formaldehyde assimilation cycle in Escherichia coli," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43610-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.