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Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment

Author

Listed:
  • Jin-Sung Park

    (Institute for Basic Science)

  • Il-Buem Lee

    (Institute for Basic Science)

  • Hyeon-Min Moon

    (Institute for Basic Science)

  • Seok-Cheol Hong

    (Institute for Basic Science
    Korea University)

  • Minhaeng Cho

    (Institute for Basic Science
    Korea University)

Abstract

A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world.

Suggested Citation

  • Jin-Sung Park & Il-Buem Lee & Hyeon-Min Moon & Seok-Cheol Hong & Minhaeng Cho, 2023. "Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42347-7
    DOI: 10.1038/s41467-023-42347-7
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    References listed on IDEAS

    as
    1. Marek Piliarik & Vahid Sandoghdar, 2014. "Direct optical sensing of single unlabelled proteins and super-resolution imaging of their binding sites," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    2. Pakorn Kanchanawong & Gleb Shtengel & Ana M. Pasapera & Ericka B. Ramko & Michael W. Davidson & Harald F. Hess & Clare M. Waterman, 2010. "Nanoscale architecture of integrin-based cell adhesions," Nature, Nature, vol. 468(7323), pages 580-584, November.
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