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Nanoscale architecture of integrin-based cell adhesions

Author

Listed:
  • Pakorn Kanchanawong

    (National Heart Lung and Blood Institute, National Institutes of Health)

  • Gleb Shtengel

    (Howard Hughes Medical Institute, Janelia Farm Research Campus)

  • Ana M. Pasapera

    (National Heart Lung and Blood Institute, National Institutes of Health)

  • Ericka B. Ramko

    (National High Magnetic Field Laboratory, The Florida State University)

  • Michael W. Davidson

    (National High Magnetic Field Laboratory, The Florida State University
    The Florida State University)

  • Harald F. Hess

    (Howard Hughes Medical Institute, Janelia Farm Research Campus)

  • Clare M. Waterman

    (National Heart Lung and Blood Institute, National Institutes of Health)

Abstract

The architecture of focal adhesions The physical linkage between the extracellular matrix and the actin cytoskeleton of a cell is made by structures known as focal adhesions, acting through integrin receptors. They are of fundamental importance in human physiology because they mediate cell adhesion, mechanosensing and signalling for the control of cell growth and differentiation. The molecular architecture of focal adhesions has now been determined using three-dimensional super-resolution fluorescence microscopy to map protein organization at the nanoscale level. They are revealed as well-organized ultrastructures in which integrins and actin are separated by a 40-nanometre-long core consisting of partially overlapping protein-specific layers, spanned by talin tethers. The multilaminar architecture creates three or more separate compartments that mediate the interdependent functions of focal adhesions.

Suggested Citation

  • Pakorn Kanchanawong & Gleb Shtengel & Ana M. Pasapera & Ericka B. Ramko & Michael W. Davidson & Harald F. Hess & Clare M. Waterman, 2010. "Nanoscale architecture of integrin-based cell adhesions," Nature, Nature, vol. 468(7323), pages 580-584, November.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7323:d:10.1038_nature09621
    DOI: 10.1038/nature09621
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    Cited by:

    1. Tianchi Chen & Cecilia H. Fernández-Espartero & Abigail Illand & Ching-Ting Tsai & Yang Yang & Benjamin Klapholz & Pierre Jouchet & Mélanie Fabre & Olivier Rossier & Bianxiao Cui & Sandrine Lévêque-Fo, 2024. "Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Sawako Yamashiro & David M. Rutkowski & Kelli Ann Lynch & Ying Liu & Dimitrios Vavylonis & Naoki Watanabe, 2023. "Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Weimin Li & Angdi Li & Bing Yu & Xiaoxiao Zhang & Xiaoyan Liu & Kate L. White & Raymond C. Stevens & Wolfgang Baumeister & Andrej Sali & Marion Jasnin & Liping Sun, 2024. "In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    4. Ronald Springer & Alexander Zielinski & Catharina Pleschka & Bernd Hoffmann & Rudolf Merkel, 2019. "Unbiased pattern analysis reveals highly diverse responses of cytoskeletal systems to cyclic straining," PLOS ONE, Public Library of Science, vol. 14(3), pages 1-23, March.
    5. J. Cody Herron & Shiqiong Hu & Takashi Watanabe & Ana T. Nogueira & Bei Liu & Megan E. Kern & Jesse Aaron & Aaron Taylor & Michael Pablo & Teng-Leong Chew & Timothy C. Elston & Klaus M. Hahn, 2022. "Actin nano-architecture of phagocytic podosomes," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    6. Xiaoyu Shi & Galo Garcia III & Yina Wang & Jeremy F Reiter & Bo Huang, 2019. "Deformed alignment of super-resolution images for semi-flexible structures," PLOS ONE, Public Library of Science, vol. 14(3), pages 1-12, March.
    7. Cecile O Mejean & Andrew W Schaefer & Kenneth B Buck & Holger Kress & Alla Shundrovsky & Jason W Merrill & Eric R Dufresne & Paul Forscher, 2013. "Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks," PLOS ONE, Public Library of Science, vol. 8(9), pages 1-1, September.
    8. Venkat R. Chirasani & Mohammad Ashhar I. Khan & Juilee N. Malavade & Nikolay V. Dokholyan & Brenton D. Hoffman & Sharon L. Campbell, 2023. "Molecular basis and cellular functions of vinculin-actin directional catch bonding," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    9. Thomas Litschel & Charlotte F. Kelley & Xiaohang Cheng & Leon Babl & Naoko Mizuno & Lindsay B. Case & Petra Schwille, 2024. "Membrane-induced 2D phase separation of the focal adhesion protein talin," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    10. Jin-Sung Park & Il-Buem Lee & Hyeon-Min Moon & Seok-Cheol Hong & Minhaeng Cho, 2023. "Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    11. Reena Kumari & Katharina Ven & Megan Chastney & Shrikant B. Kokate & Johan Peränen & Jesse Aaron & Konstantin Kogan & Leonardo Almeida-Souza & Elena Kremneva & Renaud Poincloux & Teng-Leong Chew & Pet, 2024. "Focal adhesions contain three specialized actin nanoscale layers," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    12. Alex M Valm & Rudolf Oldenbourg & Gary G Borisy, 2016. "Multiplexed Spectral Imaging of 120 Different Fluorescent Labels," PLOS ONE, Public Library of Science, vol. 11(7), pages 1-17, July.

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