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Structure of native chromatin fibres revealed by Cryo-ET in situ

Author

Listed:
  • Zhen Hou

    (University of Oxford)

  • Frank Nightingale

    (University of Oxford)

  • Yanan Zhu

    (University of Oxford)

  • Craig MacGregor-Chatwin

    (Diamond Light Source, Harwell Science and Innovation Campus)

  • Peijun Zhang

    (University of Oxford
    Diamond Light Source, Harwell Science and Innovation Campus
    University of Oxford)

Abstract

The structure of chromatin plays pivotal roles in regulating gene transcription, DNA replication and repair, and chromosome segregation. This structure, however, remains elusive. Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interphase human T-lymphoblasts and determine the in situ structures of nucleosomes in different conformations. These chromatin fibres are not structured as uniform 30 nm one-start or two-start filaments but are composed of relaxed, variable zigzag organizations of nucleosomes connected by straight linker DNA. Nucleosomes with little H1 and linker DNA density are distributed randomly without any spatial preference. This work will inspire future high-resolution investigations on native chromatin structures in situ at both a single-nucleosome level and a population level under many different cellular conditions in health and disease.

Suggested Citation

  • Zhen Hou & Frank Nightingale & Yanan Zhu & Craig MacGregor-Chatwin & Peijun Zhang, 2023. "Structure of native chromatin fibres revealed by Cryo-ET in situ," Nature Communications, Nature, vol. 14(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42072-1
    DOI: 10.1038/s41467-023-42072-1
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    References listed on IDEAS

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