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Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins

Author

Listed:
  • Namrata Kumar

    (University of Pittsburgh School of Medicine
    UPMC Hillman Cancer Center)

  • Arjan F. Theil

    (University Medical Center Rotterdam, Dr. Molewaterplein 40)

  • Vera Roginskaya

    (UPMC Hillman Cancer Center
    University of Pittsburgh School of Medicine)

  • Yasmin Ali

    (University of Pittsburgh School of Medicine)

  • Michael Calderon

    (University of Pittsburgh)

  • Simon C. Watkins

    (University of Pittsburgh)

  • Ryan P. Barnes

    (UPMC Hillman Cancer Center
    University of Pittsburgh Graduate School of Public Health)

  • Patricia L. Opresko

    (UPMC Hillman Cancer Center
    University of Pittsburgh Graduate School of Public Health)

  • Alex Pines

    (University Medical Center Rotterdam, Dr. Molewaterplein 40)

  • Hannes Lans

    (University Medical Center Rotterdam, Dr. Molewaterplein 40)

  • Wim Vermeulen

    (University Medical Center Rotterdam, Dr. Molewaterplein 40)

  • Bennett Houten

    (University of Pittsburgh School of Medicine
    UPMC Hillman Cancer Center
    University of Pittsburgh School of Medicine)

Abstract

UV-DDB, consisting of subunits DDB1 and DDB2, recognizes UV-induced photoproducts during global genome nucleotide excision repair (GG-NER). We recently demonstrated a noncanonical role of UV-DDB in stimulating base excision repair (BER) which raised several questions about the timing of UV-DDB arrival at 8-oxoguanine (8-oxoG), and the dependency of UV-DDB on the recruitment of downstream BER and NER proteins. Using two different approaches to introduce 8-oxoG in cells, we show that DDB2 is recruited to 8-oxoG immediately after damage and colocalizes with 8-oxoG glycosylase (OGG1) at sites of repair. 8-oxoG removal and OGG1 recruitment is significantly reduced in the absence of DDB2. NER proteins, XPA and XPC, also accumulate at 8-oxoG. While XPC recruitment is dependent on DDB2, XPA recruitment is DDB2-independent and transcription-coupled. Finally, DDB2 accumulation at 8-oxoG induces local chromatin unfolding. We propose that DDB2-mediated chromatin decompaction facilitates the recruitment of downstream BER proteins to 8-oxoG lesions.

Suggested Citation

  • Namrata Kumar & Arjan F. Theil & Vera Roginskaya & Yasmin Ali & Michael Calderon & Simon C. Watkins & Ryan P. Barnes & Patricia L. Opresko & Alex Pines & Hannes Lans & Wim Vermeulen & Bennett Houten, 2022. "Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28642-9
    DOI: 10.1038/s41467-022-28642-9
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    1. Aris A. Polyzos & Ana Cheong & Jung Hyun Yoo & Lana Blagec & Sneh M. Toprani & Zachary D. Nagel & Cynthia T. McMurray, 2024. "Base excision repair and double strand break repair cooperate to modulate the formation of unrepaired double strand breaks in mouse brain," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Shaqraa Musawi & Lise-Marie Donnio & Zehui Zhao & Charlène Magnani & Phoebe Rassinoux & Olivier Binda & Jianbo Huang & Arnaud Jacquier & Laurent Coudert & Patrick Lomonte & Cécile Martinat & Laurent S, 2023. "Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Brittany N. Vandenberg & Marian F. Laughery & Cameron Cordero & Dalton Plummer & Debra Mitchell & Jordan Kreyenhagen & Fatimah Albaqshi & Alexander J. Brown & Piotr A. Mieczkowski & John J. Wyrick & S, 2023. "Contributions of replicative and translesion DNA polymerases to mutagenic bypass of canonical and atypical UV photoproducts," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Athanasios Siametis & Kalliopi Stratigi & Despoina Giamaki & Georgia Chatzinikolaou & Alexia Akalestou-Clocher & Evi Goulielmaki & Brian Luke & Björn Schumacher & George A. Garinis, 2024. "Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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