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PICH acts as a force-dependent nucleosome remodeler

Author

Listed:
  • Dian Spakman

    (Vrije Universiteit Amsterdam)

  • Tinka V. M. Clement

    (Vrije Universiteit Amsterdam)

  • Andreas S. Biebricher

    (Vrije Universiteit Amsterdam)

  • Graeme A. King

    (Vrije Universiteit Amsterdam
    University College London)

  • Manika I. Singh

    (University of Copenhagen)

  • Ian D. Hickson

    (University of Copenhagen)

  • Erwin J. G. Peterman

    (Vrije Universiteit Amsterdam)

  • Gijs J. L. Wuite

    (Vrije Universiteit Amsterdam)

Abstract

In anaphase, any unresolved DNA entanglements between the segregating sister chromatids can give rise to chromatin bridges. To prevent genome instability, chromatin bridges must be resolved prior to cytokinesis. The SNF2 protein PICH has been proposed to play a direct role in this process through the remodeling of nucleosomes. However, direct evidence of nucleosome remodeling by PICH has remained elusive. Here, we present an in vitro single-molecule assay that mimics chromatin under tension, as is found in anaphase chromatin bridges. Applying a combination of dual-trap optical tweezers and fluorescence imaging of PICH and histones bound to a nucleosome-array construct, we show that PICH is a tension- and ATP-dependent nucleosome remodeler that facilitates nucleosome unwrapping and then subsequently slides remaining histones along the DNA. This work elucidates the role of PICH in chromatin-bridge dissolution, and might provide molecular insights into the mechanisms of related SNF2 proteins.

Suggested Citation

  • Dian Spakman & Tinka V. M. Clement & Andreas S. Biebricher & Graeme A. King & Manika I. Singh & Ian D. Hickson & Erwin J. G. Peterman & Gijs J. L. Wuite, 2022. "PICH acts as a force-dependent nucleosome remodeler," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35040-8
    DOI: 10.1038/s41467-022-35040-8
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    References listed on IDEAS

    as
    1. Christian F. Nielsen & Diana Huttner & Anna H. Bizard & Seiki Hirano & Tian-Neng Li & Timea Palmai-Pallag & Victoria A. Bjerregaard & Ying Liu & Erich A. Nigg & Lily Hui-Ching Wang & Ian D. Hickson, 2015. "PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis," Nature Communications, Nature, vol. 6(1), pages 1-15, December.
    2. Karolin Luger & Armin W. Mäder & Robin K. Richmond & David F. Sargent & Timothy J. Richmond, 1997. "Crystal structure of the nucleosome core particle at 2.8 Å resolution," Nature, Nature, vol. 389(6648), pages 251-260, September.
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