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Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus

Author

Listed:
  • Vincent Rincheval

    (Université de Versailles St. Quentin)

  • Mickael Lelek

    (Institut Pasteur Unité Imagerie et Modélisation)

  • Elyanne Gault

    (Université de Versailles St. Quentin
    Hôpital Ambroise Paré)

  • Camille Bouillier

    (Université de Versailles St. Quentin)

  • Delphine Sitterlin

    (Université de Versailles St. Quentin)

  • Sabine Blouquit-Laye

    (Université de Versailles St. Quentin)

  • Marie Galloux

    (Université Paris-Saclay)

  • Christophe Zimmer

    (Institut Pasteur Unité Imagerie et Modélisation)

  • Jean-François Eleouet

    (Université Paris-Saclay)

  • Marie-Anne Rameix-Welti

    (Université de Versailles St. Quentin
    Hôpital Ambroise Paré)

Abstract

Infection of cells by respiratory syncytial virus induces the formation of cytoplasmic inclusion bodies (IBs) where all the components of the viral RNA polymerase complex are concentrated. However, the exact organization and function of these IBs remain unclear. In this study, we use conventional and super-resolution imaging to dissect the internal structure of IBs. We observe that newly synthetized viral mRNA and the viral transcription anti-terminator M2-1 concentrate in IB sub-compartments, which we term “IB-associated granules” (IBAGs). In contrast, viral genomic RNA, the nucleoprotein, the L polymerase and its cofactor P are excluded from IBAGs. Live imaging reveals that IBAGs are highly dynamic structures. Our data show that IBs are the main site of viral RNA synthesis. They further suggest that shortly after synthesis in IBs, viral mRNAs and M2-1 transiently concentrate in IBAGs before reaching the cytosol and suggest a novel post-transcriptional function for M2-1.

Suggested Citation

  • Vincent Rincheval & Mickael Lelek & Elyanne Gault & Camille Bouillier & Delphine Sitterlin & Sabine Blouquit-Laye & Marie Galloux & Christophe Zimmer & Jean-François Eleouet & Marie-Anne Rameix-Welti, 2017. "Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00655-9
    DOI: 10.1038/s41467-017-00655-9
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    Cited by:

    1. Bryan S. Sibert & Joseph Y. Kim & Jie E. Yang & Zunlong Ke & Christopher C. Stobart & Martin L. Moore & Elizabeth R. Wright, 2024. "Assembly of respiratory syncytial virus matrix protein lattice and its coordination with fusion glycoprotein trimers," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Lorène Gonnin & Ambroise Desfosses & Maria Bacia-Verloop & Didier Chevret & Marie Galloux & Jean-François Éléouët & Irina Gutsche, 2023. "Structural landscape of the respiratory syncytial virus nucleocapsids," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Jack D. Whitehead & Hortense Decool & Cédric Leyrat & Loic Carrique & Jenna Fix & Jean-François Eléouët & Marie Galloux & Max Renner, 2023. "Structure of the N-RNA/P interface indicates mode of L/P recruitment to the nucleocapsid of human metapneumovirus," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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