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Structural insight into Marburg virus nucleoprotein–RNA complex formation

Author

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  • Yoko Fujita-Fujiharu

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

  • Yukihiko Sugita

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    Hakubi Center for Advanced Research, Kyoto University)

  • Yuki Takamatsu

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    National Institute of Infectious Diseases)

  • Kazuya Houri

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

  • Manabu Igarashi

    (International Institute for Zoonosis Control, Hokkaido University)

  • Yukiko Muramoto

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

  • Masahiro Nakano

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

  • Yugo Tsunoda

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

  • Ichiro Taniguchi

    (Laboratory of RNA system, Institute for Frontier Life and Medical Sciences, Kyoto University)

  • Stephan Becker

    (Institute of Virology, University of Marburg
    German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, University of Marburg)

  • Takeshi Noda

    (Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University
    Laboratory of Ultrastructural Virology, Graduate School of Biostudies, Kyoto University
    CREST, Japan Science and Technology Agency)

Abstract

The nucleoprotein (NP) of Marburg virus (MARV), a close relative of Ebola virus (EBOV), encapsidates the single-stranded, negative-sense viral genomic RNA (vRNA) to form the helical NP–RNA complex. The NP–RNA complex constitutes the core structure for the assembly of the nucleocapsid that is responsible for viral RNA synthesis. Although appropriate interactions among NPs and RNA are required for the formation of nucleocapsid, the structural basis of the helical assembly remains largely elusive. Here, we show the structure of the MARV NP–RNA complex determined using cryo-electron microscopy at a resolution of 3.1 Å. The structures of the asymmetric unit, a complex of an NP and six RNA nucleotides, was very similar to that of EBOV, suggesting that both viruses share common mechanisms for the nucleocapsid formation. Structure-based mutational analysis of both MARV and EBOV NPs identified key residues for helical assembly and subsequent viral RNA synthesis. Importantly, most of the residues identified were conserved in both viruses. These findings provide a structural basis for understanding the nucleocapsid formation and contribute to the development of novel antivirals against MARV and EBOV.

Suggested Citation

  • Yoko Fujita-Fujiharu & Yukihiko Sugita & Yuki Takamatsu & Kazuya Houri & Manabu Igarashi & Yukiko Muramoto & Masahiro Nakano & Yugo Tsunoda & Ichiro Taniguchi & Stephan Becker & Takeshi Noda, 2022. "Structural insight into Marburg virus nucleoprotein–RNA complex formation," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28802-x
    DOI: 10.1038/s41467-022-28802-x
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    References listed on IDEAS

    as
    1. Yukihiko Sugita & Hideyuki Matsunami & Yoshihiro Kawaoka & Takeshi Noda & Matthias Wolf, 2018. "Cryo-EM structure of the Ebola virus nucleoprotein–RNA complex at 3.6 Å resolution," Nature, Nature, vol. 563(7729), pages 137-140, November.
    2. William Wan & Larissa Kolesnikova & Mairi Clarke & Alexander Koehler & Takeshi Noda & Stephan Becker & John A. G. Briggs, 2017. "Structure and assembly of the Ebola virus nucleocapsid," Nature, Nature, vol. 551(7680), pages 394-397, November.
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    Cited by:

    1. Lorène Gonnin & Ambroise Desfosses & Maria Bacia-Verloop & Didier Chevret & Marie Galloux & Jean-François Éléouët & Irina Gutsche, 2023. "Structural landscape of the respiratory syncytial virus nucleocapsids," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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