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Cryo-EM structure of single-layered nucleoprotein-RNA complex from Marburg virus

Author

Listed:
  • Luca Zinzula

    (Research Group Molecular Structural Biology
    ShanghaiTech University)

  • Florian Beck

    (Research Group Molecular Structural Biology
    Research Group CryoEM Technology)

  • Marianna Camasta

    (Research Group Molecular Structural Biology)

  • Stefan Bohn

    (Research Group Molecular Structural Biology
    Helmholtz Center Munich)

  • Chuan Liu

    (Research Group Molecular Structural Biology)

  • Dustin Morado

    (Department of Cell and Virus Structure
    Science for Life Laboratory)

  • Andreas Bracher

    (Department of Cellular Biochemistry)

  • Juergen M. Plitzko

    (Research Group Molecular Structural Biology
    Research Group CryoEM Technology)

  • Wolfgang Baumeister

    (Research Group Molecular Structural Biology
    ShanghaiTech University)

Abstract

Marburg virus (MARV) causes lethal hemorrhagic fever in humans, posing a threat to global health. We determined by cryogenic electron microscopy (cryo-EM) the MARV helical ribonucleoprotein (RNP) complex structure in single-layered conformation, which differs from the previously reported structure of a double-layered helix. Our findings illuminate novel RNP interactions and expand knowledge on MARV genome packaging and nucleocapsid assembly, both processes representing attractive targets for the development of antiviral therapeutics against MARV disease.

Suggested Citation

  • Luca Zinzula & Florian Beck & Marianna Camasta & Stefan Bohn & Chuan Liu & Dustin Morado & Andreas Bracher & Juergen M. Plitzko & Wolfgang Baumeister, 2024. "Cryo-EM structure of single-layered nucleoprotein-RNA complex from Marburg virus," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54431-7
    DOI: 10.1038/s41467-024-54431-7
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    References listed on IDEAS

    as
    1. Yukihiko Sugita & Hideyuki Matsunami & Yoshihiro Kawaoka & Takeshi Noda & Matthias Wolf, 2018. "Cryo-EM structure of the Ebola virus nucleoprotein–RNA complex at 3.6 Å resolution," Nature, Nature, vol. 563(7729), pages 137-140, November.
    2. William Wan & Larissa Kolesnikova & Mairi Clarke & Alexander Koehler & Takeshi Noda & Stephan Becker & John A. G. Briggs, 2017. "Structure and assembly of the Ebola virus nucleocapsid," Nature, Nature, vol. 551(7680), pages 394-397, November.
    3. Yoko Fujita-Fujiharu & Yukihiko Sugita & Yuki Takamatsu & Kazuya Houri & Manabu Igarashi & Yukiko Muramoto & Masahiro Nakano & Yugo Tsunoda & Ichiro Taniguchi & Stephan Becker & Takeshi Noda, 2022. "Structural insight into Marburg virus nucleoprotein–RNA complex formation," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
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