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Cryo-EM structure of a bacteriophage M13 mini variant

Author

Listed:
  • Qi Jia

    (Tsinghua University)

  • Ye Xiang

    (Tsinghua University)

Abstract

Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.

Suggested Citation

  • Qi Jia & Ye Xiang, 2023. "Cryo-EM structure of a bacteriophage M13 mini variant," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41151-7
    DOI: 10.1038/s41467-023-41151-7
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    References listed on IDEAS

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    1. Wen Jiang & Juan Chang & Joanita Jakana & Peter Weigele & Jonathan King & Wah Chiu, 2006. "Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus," Nature, Nature, vol. 439(7076), pages 612-616, February.
    2. James C. Samuelson & Minyong Chen & Fenglei Jiang & Ines Möller & Martin Wiedmann & Andreas Kuhn & Gregory J. Phillips & Ross E. Dalbey, 2000. "YidC mediates membrane protein insertion in bacteria," Nature, Nature, vol. 406(6796), pages 637-641, August.
    3. Haijuan Yang & Chun Zhou & Ankita Dhar & Nikola P. Pavletich, 2020. "Mechanism of strand exchange from RecA–DNA synaptic and D-loop structures," Nature, Nature, vol. 586(7831), pages 801-806, October.
    4. Dongjie Zhu & Xiangxi Wang & Qianglin Fang & James L Etten & Michael G Rossmann & Zihe Rao & Xinzheng Zhang, 2018. "Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
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