IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v439y2006i7076d10.1038_nature04487.html
   My bibliography  Save this article

Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus

Author

Listed:
  • Wen Jiang

    (National Center for Macromolecular Imaging
    Purdue University)

  • Juan Chang

    (National Center for Macromolecular Imaging
    Baylor College of Medicine)

  • Joanita Jakana

    (National Center for Macromolecular Imaging)

  • Peter Weigele

    (Massachusetts Institute of Technology)

  • Jonathan King

    (Massachusetts Institute of Technology)

  • Wah Chiu

    (National Center for Macromolecular Imaging
    Baylor College of Medicine)

Abstract

Spherical virus structure Structural determinations of spherical viruses have so far been limited to the capsid shell with icosahedral symmetry. Now for the first time an entire spherical virus structure has been determined without imposing icosahedral symmetry, a strategy that was necessary to simplify image reconstruction calculations. The virus in question is epsilon15 phage, which infects the human pathogen Salmonella anatum so is a potential therapeutic agent for salmonellosis. Single-particle cryo-electron microscopy shows the icosahedral protein shell to consist of 60 hexamers and 11 pentamers. Non-icosahedral components cluster at one of the twelve capsid vertices, through which DNA is packaged and released. The genome is packed in coaxial coils and a previously unidentified protein core wraps around the terminal end of the DNA. The shell resembles those of other dsDNA viruses including herpesvirus, suggesting a common ancestor.

Suggested Citation

  • Wen Jiang & Juan Chang & Joanita Jakana & Peter Weigele & Jonathan King & Wah Chiu, 2006. "Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus," Nature, Nature, vol. 439(7076), pages 612-616, February.
    • Handle: RePEc:nat:nature:v:439:y:2006:i:7076:d:10.1038_nature04487
    DOI: 10.1038/nature04487
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04487
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04487?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Qi Jia & Ye Xiang, 2023. "Cryo-EM structure of a bacteriophage M13 mini variant," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Xudong Jia & Yuanzhu Gao & Yuxuan Huang & Linjun Sun & Siduo Li & Hongmei Li & Xueqing Zhang & Yinyin Li & Jian He & Wenbi Wu & Harikanth Venkannagari & Kai Yang & Matthew L. Baker & Qinfen Zhang, 2023. "Architecture of the baculovirus nucleocapsid revealed by cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Marta Šiborová & Tibor Füzik & Michaela Procházková & Jiří Nováček & Martin Benešík & Anders S. Nilsson & Pavel Plevka, 2022. "Tail proteins of phage SU10 reorganize into the nozzle for genome delivery," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:439:y:2006:i:7076:d:10.1038_nature04487. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.