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Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus

Author

Listed:
  • Wen Jiang

    (National Center for Macromolecular Imaging
    Purdue University)

  • Juan Chang

    (National Center for Macromolecular Imaging
    Baylor College of Medicine)

  • Joanita Jakana

    (National Center for Macromolecular Imaging)

  • Peter Weigele

    (Massachusetts Institute of Technology)

  • Jonathan King

    (Massachusetts Institute of Technology)

  • Wah Chiu

    (National Center for Macromolecular Imaging
    Baylor College of Medicine)

Abstract

Spherical virus structure Structural determinations of spherical viruses have so far been limited to the capsid shell with icosahedral symmetry. Now for the first time an entire spherical virus structure has been determined without imposing icosahedral symmetry, a strategy that was necessary to simplify image reconstruction calculations. The virus in question is epsilon15 phage, which infects the human pathogen Salmonella anatum so is a potential therapeutic agent for salmonellosis. Single-particle cryo-electron microscopy shows the icosahedral protein shell to consist of 60 hexamers and 11 pentamers. Non-icosahedral components cluster at one of the twelve capsid vertices, through which DNA is packaged and released. The genome is packed in coaxial coils and a previously unidentified protein core wraps around the terminal end of the DNA. The shell resembles those of other dsDNA viruses including herpesvirus, suggesting a common ancestor.

Suggested Citation

  • Wen Jiang & Juan Chang & Joanita Jakana & Peter Weigele & Jonathan King & Wah Chiu, 2006. "Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus," Nature, Nature, vol. 439(7076), pages 612-616, February.
    • Handle: RePEc:nat:nature:v:439:y:2006:i:7076:d:10.1038_nature04487
    DOI: 10.1038/nature04487
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    Cited by:

    1. Qi Jia & Ye Xiang, 2023. "Cryo-EM structure of a bacteriophage M13 mini variant," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Marta Šiborová & Tibor Füzik & Michaela Procházková & Jiří Nováček & Martin Benešík & Anders S. Nilsson & Pavel Plevka, 2022. "Tail proteins of phage SU10 reorganize into the nozzle for genome delivery," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Xudong Jia & Yuanzhu Gao & Yuxuan Huang & Linjun Sun & Siduo Li & Hongmei Li & Xueqing Zhang & Yinyin Li & Jian He & Wenbi Wu & Harikanth Venkannagari & Kai Yang & Matthew L. Baker & Qinfen Zhang, 2023. "Architecture of the baculovirus nucleocapsid revealed by cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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