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Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly

Author

Listed:
  • Zhennan Zhao

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Youhua Huang

    (South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture)

  • Congcong Liu

    (Southern University of Science and Technology
    Shenzhen Third People’s Hospital)

  • Dongjie Zhu

    (University of Science and Technology of China
    Institute of Biophysics, Chinese Academy of Sciences)

  • Shuaixin Gao

    (Chinese Academy of Medical Science & Peking Union Medical College
    The Ohio State University)

  • Sheng Liu

    (Southern University of Science and Technology)

  • Ruchao Peng

    (Institute of Microbiology, Chinese Academy of Sciences
    University of Pennsylvania)

  • Ya Zhang

    (South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture)

  • Xiaohong Huang

    (South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture)

  • Jianxun Qi

    (Institute of Microbiology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Beijing Life Science Academy)

  • Catherine C. L. Wong

    (Chinese Academy of Medical Science & Peking Union Medical College)

  • Xinzheng Zhang

    (Institute of Biophysics, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Peiyi Wang

    (Southern University of Science and Technology)

  • Qiwei Qin

    (South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture)

  • George F. Gao

    (Institute of Microbiology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Singapore grouper iridovirus (SGIV), one of the nucleocytoviricota viruses (NCVs), is a highly pathogenic iridovirid. SGIV infection results in massive economic losses to the aquaculture industry and significantly threatens global biodiversity. In recent years, high morbidity and mortality in aquatic animals have been caused by iridovirid infections worldwide. Effective control and prevention strategies are urgently needed. Here, we present a near-atomic architecture of the SGIV capsid and identify eight types of capsid proteins. The viral inner membrane-integrated anchor protein colocalizes with the endoplasmic reticulum (ER), supporting the hypothesis that the biogenesis of the inner membrane is associated with the ER. Additionally, immunofluorescence assays indicate minor capsid proteins (mCPs) could form various building blocks with major capsid proteins (MCPs) before the formation of a viral factory (VF). These results expand our understanding of the capsid assembly of NCVs and provide more targets for vaccine and drug design to fight iridovirid infections.

Suggested Citation

  • Zhennan Zhao & Youhua Huang & Congcong Liu & Dongjie Zhu & Shuaixin Gao & Sheng Liu & Ruchao Peng & Ya Zhang & Xiaohong Huang & Jianxun Qi & Catherine C. L. Wong & Xinzheng Zhang & Peiyi Wang & Qiwei , 2023. "Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37681-9
    DOI: 10.1038/s41467-023-37681-9
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    References listed on IDEAS

    as
    1. Qianglin Fang & Dongjie Zhu & Irina Agarkova & Jagat Adhikari & Thomas Klose & Yue Liu & Zhenguo Chen & Yingyuan Sun & Michael L. Gross & James L. Van Etten & Xinzheng Zhang & Michael G. Rossmann, 2019. "Near-atomic structure of a giant virus," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    2. Dongjie Zhu & Xiangxi Wang & Qianglin Fang & James L Etten & Michael G Rossmann & Zihe Rao & Xinzheng Zhang, 2018. "Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
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