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YidC mediates membrane protein insertion in bacteria

Author

Listed:
  • James C. Samuelson

    (The Ohio State University)

  • Minyong Chen

    (The Ohio State University)

  • Fenglei Jiang

    (The Ohio State University)

  • Ines Möller

    (Cellular Biochemistry and Biophysic Program, Memorial Sloan-Kettering Cancer Centre)

  • Martin Wiedmann

    (Cellular Biochemistry and Biophysic Program, Memorial Sloan-Kettering Cancer Centre)

  • Andreas Kuhn

    (University of Hohenheim, Institute for Microbiology and Molecular Biology)

  • Gregory J. Phillips

    (Iowa State University)

  • Ross E. Dalbey

    (The Ohio State University)

Abstract

The basic machinery for the translocation of proteins into or across membranes is remarkably conserved from Escherichia coli to humans. In eukaryotes, proteins are inserted into the endoplasmic reticulum using the signal recognition particle (SRP) and the SRP receptor, as well as the integral membrane Sec61 trimeric complex (composed of alpha, beta and gamma subunits)1. In bacteria, most proteins are inserted by a related pathway that includes the SRP homologue Ffh2,3,4,5, the SRP receptor FtsY6,7, and the SecYEG trimeric complex8, where Y and E are related to the Sec61 alpha and gamma subunits, respectively. Proteins in bacteria that exhibit no dependence on the Sec translocase were previously thought to insert into the membrane directly without the aid of a protein machinery9,10. Here we show that membrane insertion of two Sec-independent proteins requires YidC. YidC is essential for E. coli viability and homologues are present in mitochondria and chloroplasts. Depletion of YidC also interferes with insertion of Sec-dependent membrane proteins, but it has only a minor effect on the export of secretory proteins. These results provide evidence for an additional component of the translocation machinery that is specialized for the integration of membrane proteins.

Suggested Citation

  • James C. Samuelson & Minyong Chen & Fenglei Jiang & Ines Möller & Martin Wiedmann & Andreas Kuhn & Gregory J. Phillips & Ross E. Dalbey, 2000. "YidC mediates membrane protein insertion in bacteria," Nature, Nature, vol. 406(6796), pages 637-641, August.
    • Handle: RePEc:nat:nature:v:406:y:2000:i:6796:d:10.1038_35020586
    DOI: 10.1038/35020586
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    Cited by:

    1. Qi Jia & Ye Xiang, 2023. "Cryo-EM structure of a bacteriophage M13 mini variant," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Pawel R. Laskowski & Kristyna Pluhackova & Maximilian Haase & Brian M. Lang & Gisela Nagler & Andreas Kuhn & Daniel J. Müller, 2021. "Monitoring the binding and insertion of a single transmembrane protein by an insertase," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

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