IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-36503-2.html
   My bibliography  Save this article

Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

Author

Listed:
  • Anne Edwards

    (John Innes Centre, Norwich Research Park)

  • Isaac Njaci

    (John Innes Centre, Norwich Research Park
    Biosciences eastern and central Africa International Livestock Research Institute Hub
    Queensland University of Technology)

  • Abhimanyu Sarkar

    (John Innes Centre, Norwich Research Park
    National Institute of Agricultural Botany)

  • Zhouqian Jiang

    (John Innes Centre, Norwich Research Park
    Capital Medical University, You An Men)

  • Gemy George Kaithakottil

    (Earlham Institute, Norwich Research Park, Colney Lane)

  • Christopher Moore

    (School of Life Sciences, University of Nottingham, University Park)

  • Jitender Cheema

    (John Innes Centre, Norwich Research Park)

  • Clare E. M. Stevenson

    (John Innes Centre, Norwich Research Park)

  • Martin Rejzek

    (John Innes Centre, Norwich Research Park)

  • Petr Novák

    (Institute of Plant Molecular Biology, Biology Centre CAS)

  • Marielle Vigouroux

    (John Innes Centre, Norwich Research Park)

  • Martin Vickers

    (John Innes Centre, Norwich Research Park)

  • Roland H. M. Wouters

    (John Innes Centre, Norwich Research Park)

  • Pirita Paajanen

    (John Innes Centre, Norwich Research Park)

  • Burkhard Steuernagel

    (John Innes Centre, Norwich Research Park)

  • Jonathan D. Moore

    (John Innes Centre, Norwich Research Park)

  • Janet Higgins

    (Earlham Institute, Norwich Research Park, Colney Lane)

  • David Swarbreck

    (Earlham Institute, Norwich Research Park, Colney Lane)

  • Stefan Martens

    (Research and Innovation Centre, Fondazione Edmund Mach)

  • Colin Y. Kim

    (Whitehead Institute for Biomedical Research
    Massachusetts Institute of Technology)

  • Jing-Ke Weng

    (Whitehead Institute for Biomedical Research
    Massachusetts Institute of Technology)

  • Sagadevan Mundree

    (Queensland University of Technology)

  • Benjamin Kilian

    (Global Crop Diversity Trust)

  • Shiv Kumar

    (International Center for Agricultural Research in the Dry Areas, Avenue Hafiane Cherkaoui)

  • Matt Loose

    (School of Life Sciences, University of Nottingham, University Park)

  • Levi Yant

    (School of Life Sciences, University of Nottingham, University Park
    University of Nottingham)

  • Jiří Macas

    (Institute of Plant Molecular Biology, Biology Centre CAS)

  • Trevor L. Wang

    (John Innes Centre, Norwich Research Park)

  • Cathie Martin

    (John Innes Centre, Norwich Research Park)

  • Peter M. F. Emmrich

    (John Innes Centre, Norwich Research Park
    Biosciences eastern and central Africa International Livestock Research Institute Hub
    University of East Anglia)

Abstract

Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.

Suggested Citation

  • Anne Edwards & Isaac Njaci & Abhimanyu Sarkar & Zhouqian Jiang & Gemy George Kaithakottil & Christopher Moore & Jitender Cheema & Clare E. M. Stevenson & Martin Rejzek & Petr Novák & Marielle Vigourou, 2023. "Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36503-2
    DOI: 10.1038/s41467-023-36503-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-36503-2
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-36503-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Pauline Chivenge & Tafadzwanashe Mabhaudhi & Albert T. Modi & Paramu Mafongoya, 2015. "The Potential Role of Neglected and Underutilised Crop Species as Future Crops under Water Scarce Conditions in Sub-Saharan Africa," IJERPH, MDPI, vol. 12(6), pages 1-27, May.
    2. Juliana D. B. Gil & Avery S. Cohn & John Duncan & Peter Newton & Sonja Vermeulen, 2017. "The resilience of integrated agricultural systems to climate change," Wiley Interdisciplinary Reviews: Climate Change, John Wiley & Sons, vol. 8(4), July.
    3. Mabhaudhi, T. & Chimonyo, V. G. P. & Hlahla, S. & Massawe, F. & Mayes, S. & Nhamo, Luxon & Modi, A. T., 2019. "Prospects of orphan crops in climate change," Papers published in Journals (Open Access), International Water Management Institute, pages 250(3):695-.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Oluwaseyi Shorinola & Rose Marks & Peter Emmrich & Chris Jones & Damaris Odeny & Mark A. Chapman, 2024. "Integrative and inclusive genomics to promote the use of underutilised crops," Nature Communications, Nature, vol. 15(1), pages 1-4, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Vimbayi Grace Petrova Chimonyo & Tendai Polite Chibarabada & Dennis Junior Choruma & Richard Kunz & Sue Walker & Festo Massawe & Albert Thembinkosi Modi & Tafadzwanashe Mabhaudhi, 2022. "Modelling Neglected and Underutilised Crops: A Systematic Review of Progress, Challenges, and Opportunities," Sustainability, MDPI, vol. 14(21), pages 1-19, October.
    2. Chimonyo, V. G. P. & Chibarabada, T. P. & Choruma, D. J. & Kunz, R. & Walker, S. & Massawe, F. & Modi, A. T. & Mabhaudhi, Tafadzwanashe, 2022. "Modelling neglected and underutilised crops: a systematic review of progress, challenges, and opportunities," Papers published in Journals (Open Access), International Water Management Institute, pages 1-14(21):13.
    3. Hamid El Bilali & Susanna Rokka & Generosa Calabrese & Teresa Borelli & Francesca Grazioli & Sheirita Reine Fanta Tietiambou & Jacques Nanema & Iro Dan Guimbo & Lawali Dambo & Bassirou Nouhou & Maria , 2024. "Conservation and Promotion of Neglected and Underutilized Crop Species in West Africa: Policy and Governance," Sustainability, MDPI, vol. 16(14), pages 1-20, July.
    4. Tendai Polite Chibarabada & Albert Thembinkosi Modi & Tafadzwanashe Mabhaudhi, 2017. "Nutrient Content and Nutritional Water Productivity of Selected Grain Legumes in Response to Production Environment," IJERPH, MDPI, vol. 14(11), pages 1-17, October.
    5. Sussy Munialo & Alexandros Gasparatos & Ndiko Ludidi & Ali Elnaeim Elbasheir Ali & Eden Keyster & Musa Oyebowale Akanbi & Mohammad Naushad Emmambux, 2024. "Systematic Review of the Agro-Ecological, Nutritional, and Medicinal Properties of the Neglected and Underutilized Plant Species Tylosema fassoglense," Sustainability, MDPI, vol. 16(14), pages 1-29, July.
    6. Marija Knez & Marija Ranic & Mirjana Gurinovic & Maria Glibetic & Jasna Savic & Konstadinos Mattas & Murat Yercan, 2023. "Causes and Conditions for Reduced Cultivation and Consumption of Underutilized Crops: Is There a Solution?," Sustainability, MDPI, vol. 15(4), pages 1-15, February.
    7. Bagus Setiabudi Wiwoho & Ike Sari Astuti & Purwanto Purwanto & Ifan Deffinika & Imam Abdul Gani Alfarizi & Hetty Rahmawati Sucahyo & Randhiki Gusti & Mochammad Tri Herwanto & Gilang Aulia Herlambang, 2023. "Assessing long-term rainfall trends and changes in a tropical watershed Brantas, Indonesia: an approach for quantifying the agreement among satellite-based rainfall data, ground rainfall data, and sma," Natural Hazards: Journal of the International Society for the Prevention and Mitigation of Natural Hazards, Springer;International Society for the Prevention and Mitigation of Natural Hazards, vol. 117(3), pages 2835-2862, July.
    8. Maria Gialeli & Andreas Y. Troumbis & Constantinos Giaginis & Sousana K. Papadopoulou & Ioannis Antoniadis & Georgios K. Vasios, 2023. "The Global Growth of ‘Sustainable Diet’ during Recent Decades, a Bibliometric Analysis," Sustainability, MDPI, vol. 15(15), pages 1-23, August.
    9. Dosso, Moussa & Nandjui, Jacob & Avadí, Angel, 2024. "Understanding the Ivorian market vegetables production: Is the agroecological transition the right strategy?," Agricultural Systems, Elsevier, vol. 218(C).
    10. Mengyi Zhang & Peter Dannenberg, 2022. "Opportunities and Challenges of Indigenous Food Plant Farmers in Integrating into Agri-Food Value Chains in Cape Town," Land, MDPI, vol. 11(12), pages 1-17, December.
    11. Ranasinghe, Ranawalage Dona Arani Koshathaki & Korale-Gedara, Pradeepa Malkanthi & Weerasooriya, Senal Alexander, 2023. "Climate change adaptation and adaptive capacities of dairy farmers: Evidence from village tank cascade systems in Sri Lanka," Agricultural Systems, Elsevier, vol. 206(C).
    12. Nafiisa Sobratee & Rashieda Davids & Chuma B. Chinzila & Tafadzwanashe Mabhaudhi & Pauline Scheelbeek & Albert T. Modi & Alan D. Dangour & Rob Slotow, 2022. "Visioning a Food System for an Equitable Transition towards Sustainable Diets—A South African Perspective," Sustainability, MDPI, vol. 14(6), pages 1-23, March.
    13. George Malindretos & Eleni Sardianou & Maria Briana, 2023. "Farmers’ Perception on Contract Farming in the Post-COVID Era: Empirical Study in Greece," Sustainability, MDPI, vol. 15(20), pages 1-14, October.
    14. Marine Albert & Jacques-Eric Bergez & Magali Willaume & Stéphane Couture, 2022. "Vulnerability of Maize Farming Systems to Climate Change: Farmers’ Opinions Differ about the Relevance of Adaptation Strategies," Sustainability, MDPI, vol. 14(14), pages 1-23, July.
    15. Takudzwa Mandizvo & Alfred Oduor Odindo & Jacob Mashilo, 2021. "Citron Watermelon Potential to Improve Crop Diversification and Reduce Negative Impacts of Climate Change," Sustainability, MDPI, vol. 13(4), pages 1-17, February.
    16. Hemalatha Palanivel & Shipra Shah, 2021. "Unlocking the inherent potential of plant genetic resources: food security and climate adaptation strategy in Fiji and the Pacific," Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, Springer, vol. 23(10), pages 14264-14323, October.
    17. Magidi, J. & van Koppen, Barbara & Nhamo, L. & Mpandeli, S. & Slotow, R. & Mabhaudhi, Tafadzwanashe, 2021. "Informing equitable water and food policies through accurate spatial information on irrigated areas in smallholder farming systems," Papers published in Journals (Open Access), International Water Management Institute, pages 1-13(24):36.
    18. Monde Rapiya & Wayne Truter & Abel Ramoelo, 2024. "The Integration of Land Restoration and Biodiversity Conservation Practices in Sustainable Food Systems of Africa: A Systematic Review," Sustainability, MDPI, vol. 16(20), pages 1-22, October.
    19. Karina Jiménez-Morales & Eduardo Castañeda-Pérez & Emanuel Herrera-Pool & Teresa Ayora-Talavera & Juan Carlos Cuevas-Bernardino & Ulises García-Cruz & Soledad Cecilia Pech-Cohuo & Neith Pacheco, 2022. "Ultrasound-Assisted Extraction of Phenolic Compounds from Different Maturity Stages and Fruit Parts of Cordia dodecandra A. DC.: Quantification and Identification by UPLC-DAD-ESI-MS/MS," Agriculture, MDPI, vol. 12(12), pages 1-15, December.
    20. Tafadzwanashe Mabhaudhi & Tendai Chibarabada & Albert Modi, 2016. "Water-Food-Nutrition-Health Nexus: Linking Water to Improving Food, Nutrition and Health in Sub-Saharan Africa," IJERPH, MDPI, vol. 13(1), pages 1-19, January.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36503-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.