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Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters

Author

Listed:
  • Marcos Matamoros

    (Washington University School of Medicine
    Washington University School of Medicine)

  • Xue Wen Ng

    (Washington University School of Medicine
    Washington University School of Medicine)

  • Joshua B. Brettmann

    (Washington University School of Medicine
    Millipore-Sigma Inc.)

  • David W. Piston

    (Washington University School of Medicine
    Washington University School of Medicine)

  • Colin G. Nichols

    (Washington University School of Medicine
    Washington University School of Medicine)

Abstract

The K+ channel selectivity filter (SF) is defined by TxGYG amino acid sequences that generate four identical K+ binding sites (S1-S4). Only two sites (S3, S4) are present in the non-selective bacterial NaK channel, but a four-site K+-selective SF is obtained by mutating the wild-type TVGDGN SF sequence to a canonical K+ channel TVGYGD sequence (NaK2K mutant). Using single molecule FRET (smFRET), we show that the SF of NaK2K, but not of non-selective NaK, is ion-dependent, with the constricted SF configuration stabilized in high K+ conditions. Patch-clamp electrophysiology and non-canonical fluorescent amino acid incorporation show that NaK2K selectivity is reduced by crosslinking to limit SF conformational movement. Finally, the eukaryotic K+ channel TREK2 SF exhibits essentially identical smFRET-reported ion-dependent conformations as in prokaryotic K+ channels. Our results establish the generality of K+-induced SF conformational stability across the K+ channel superfamily, and introduce an approach to study manipulation of channel selectivity.

Suggested Citation

  • Marcos Matamoros & Xue Wen Ng & Joshua B. Brettmann & David W. Piston & Colin G. Nichols, 2023. "Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-022-35756-7
    DOI: 10.1038/s41467-022-35756-7
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    as
    1. Yufeng Zhou & João H. Morais-Cabral & Amelia Kaufman & Roderick MacKinnon, 2001. "Chemistry of ion coordination and hydration revealed by a K+ channel–Fab complex at 2.0 Å resolution," Nature, Nature, vol. 414(6859), pages 43-48, November.
    2. David B. Sauer & Weizhong Zeng & John Canty & Yeeling Lam & Youxing Jiang, 2013. "Sodium and potassium competition in potassium-selective and non-selective channels," Nature Communications, Nature, vol. 4(1), pages 1-9, December.
    3. Matthew R. Whorton & Roderick MacKinnon, 2013. "X-ray structure of the mammalian GIRK2–βγ G-protein complex," Nature, Nature, vol. 498(7453), pages 190-197, June.
    4. Purushotham Selvakumar & Ana I. Fernández-Mariño & Nandish Khanra & Changhao He & Alice J. Paquette & Bing Wang & Ruiqi Huang & Vaughn V. Smider & William J. Rice & Kenton J. Swartz & Joel R. Meyerson, 2022. "Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Youxing Jiang & Alice Lee & Jiayun Chen & Vanessa Ruta & Martine Cadene & Brian T. Chait & Roderick MacKinnon, 2003. "X-ray structure of a voltage-dependent K+ channel," Nature, Nature, vol. 423(6935), pages 33-41, May.
    6. Youxing Jiang & Alice Lee & Jiayun Chen & Martine Cadene & Brian T. Chait & Roderick MacKinnon, 2002. "Crystal structure and mechanism of a calcium-gated potassium channel," Nature, Nature, vol. 417(6888), pages 515-522, May.
    7. Stephen B. Long & Xiao Tao & Ernest B. Campbell & Roderick MacKinnon, 2007. "Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment," Nature, Nature, vol. 450(7168), pages 376-382, November.
    8. Scott B. Hansen & Xiao Tao & Roderick MacKinnon, 2011. "Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2," Nature, Nature, vol. 477(7365), pages 495-498, September.
    9. Baobin Li & Robert A. Rietmeijer & Stephen G. Brohawn, 2020. "Structural basis for pH gating of the two-pore domain K+ channel TASK2," Nature, Nature, vol. 586(7829), pages 457-462, October.
    10. Fan Yang & Xian Xiao & Bo Hyun Lee & Simon Vu & Wei Yang & Vladimir Yarov-Yarovoy & Jie Zheng, 2018. "The conformational wave in capsaicin activation of transient receptor potential vanilloid 1 ion channel," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    11. Lizhen Xu & Yalan Han & Xiaoying Chen & Aerziguli Aierken & Han Wen & Wenjun Zheng & Hongkun Wang & Xiancui Lu & Zhenye Zhao & Cheng Ma & Ping Liang & Wei Yang & Shilong Yang & Fan Yang, 2020. "Molecular mechanisms underlying menthol binding and activation of TRPM8 ion channel," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    12. Han Sun & Liqun Luo & Bachchu Lal & Xinrong Ma & Lieping Chen & Christine L. Hann & Amy M. Fulton & Daniel J. Leahy & John Laterra & Min Li, 2016. "A monoclonal antibody against KCNK9 K+ channel extracellular domain inhibits tumour growth and metastasis," Nature Communications, Nature, vol. 7(1), pages 1-12, April.
    13. Adam Lewis & Vilius Kurauskas & Marco Tonelli & Katherine Henzler-Wildman, 2021. "Ion-dependent structure, dynamics, and allosteric coupling in a non-selective cation channel," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    14. Stephen G. Brohawn & Ernest B. Campbell & Roderick MacKinnon, 2014. "Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel," Nature, Nature, vol. 516(7529), pages 126-130, December.
    15. Ning Shi & Sheng Ye & Amer Alam & Liping Chen & Youxing Jiang, 2006. "Atomic structure of a Na+- and K+-conducting channel," Nature, Nature, vol. 440(7083), pages 570-574, March.
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