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Crystal structure and mechanism of a calcium-gated potassium channel

Author

Listed:
  • Youxing Jiang

    (Rockefeller University)

  • Alice Lee

    (Rockefeller University)

  • Jiayun Chen

    (Rockefeller University)

  • Martine Cadene

    (Rockefeller University)

  • Brian T. Chait

    (Rockefeller University)

  • Roderick MacKinnon

    (Rockefeller University)

Abstract

Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an appropriate stimulus. Two general categories of ion channel gating are defined by the initiating stimulus: ligand binding (neurotransmitter- or second-messenger-gated channels) or membrane voltage (voltage-gated channels). Here we present the structural basis of ligand gating in a K+ channel that opens in response to intracellular Ca2+. We have cloned, expressed, analysed electrical properties, and determined the crystal structure of a K+ channel (MthK) from Methanobacterium thermoautotrophicum in the Ca2+-bound, opened state. Eight RCK domains (regulators of K+ conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca2+ binding in a simple manner to perform mechanical work to open the pore.

Suggested Citation

  • Youxing Jiang & Alice Lee & Jiayun Chen & Martine Cadene & Brian T. Chait & Roderick MacKinnon, 2002. "Crystal structure and mechanism of a calcium-gated potassium channel," Nature, Nature, vol. 417(6888), pages 515-522, May.
    • Handle: RePEc:nat:nature:v:417:y:2002:i:6888:d:10.1038_417515a
    DOI: 10.1038/417515a
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    Cited by:

    1. Tobias Raisch & Andreas Brockmann & Ulrich Ebbinghaus-Kintscher & Jörg Freigang & Oliver Gutbrod & Jan Kubicek & Barbara Maertens & Oliver Hofnagel & Stefan Raunser, 2021. "Small molecule modulation of the Drosophila Slo channel elucidated by cryo-EM," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Eva Chovancova & Antonin Pavelka & Petr Benes & Ondrej Strnad & Jan Brezovsky & Barbora Kozlikova & Artur Gora & Vilem Sustr & Martin Klvana & Petr Medek & Lada Biedermannova & Jiri Sochor & Jiri Damb, 2012. "CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures," PLOS Computational Biology, Public Library of Science, vol. 8(10), pages 1-12, October.
    3. Marcos Matamoros & Xue Wen Ng & Joshua B. Brettmann & David W. Piston & Colin G. Nichols, 2023. "Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Yangyang Wang & Shaokang Yang & Jingwei Zhang & Zhuo Chen & Bo Zhu & Jian Li & Shijing Liang & Yunxiang Bai & Jianhong Xu & Dewei Rao & Liangliang Dong & Chunfang Zhang & Xiaowei Yang, 2023. "Scalable and switchable CO2-responsive membranes with high wettability for separation of various oil/water systems," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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