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Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation

Author

Listed:
  • Gi Uk Jeong

    (Korea Research Institute of Chemical Technology)

  • Hyung-Jun Kwon

    (Korea Research Institute of Bioscience and Biotechnology
    Jeonbuk Branch, Korea Institute of Toxicology)

  • Wern Hann Ng

    (Griffith University
    Griffith University
    Griffith University)

  • Xiang Liu

    (Griffith University
    Griffith University
    Griffith University)

  • Hyun Woo Moon

    (Korea Research Institute of Chemical Technology)

  • Gun Young Yoon

    (Korea Research Institute of Chemical Technology)

  • Hye Jin Shin

    (Korea Research Institute of Chemical Technology
    Chungnam National University School of Medicine)

  • In-Chul Lee

    (Korea Research Institute of Bioscience and Biotechnology
    Jeonbuk Branch, Korea Institute of Toxicology)

  • Zheng Lung Ling

    (The University of Sydney
    The University of Sydney)

  • Alanna G. Spiteri

    (The University of Sydney
    The University of Sydney)

  • Nicholas J. C. King

    (Griffith University
    The University of Sydney
    The University of Sydney
    The University of Sydney)

  • Adam Taylor

    (Griffith University
    Griffith University
    Griffith University)

  • Ji Soo Chae

    (PerkinElmer)

  • Chonsaeng Kim

    (Korea Research Institute of Chemical Technology)

  • Dae-Gyun Ahn

    (Korea Research Institute of Chemical Technology)

  • Kyun-Do Kim

    (Korea Research Institute of Chemical Technology)

  • Young Bae Ryu

    (Korea Research Institute of Bioscience and Biotechnology)

  • Seong-Jun Kim

    (Korea Research Institute of Chemical Technology)

  • Suresh Mahalingam

    (Griffith University
    Griffith University
    Griffith University)

  • Young-Chan Kwon

    (Korea Research Institute of Chemical Technology)

Abstract

Although ocular manifestations are reported in patients with COVID-19, consensus on ocular tropism of SARS-CoV-2 is lacking. Here, we infect K18-hACE2 transgenic mice with SARS-CoV-2 using various routes. We observe ocular manifestation and retinal inflammation with production of pro-inflammatory cytokines in the eyes of intranasally (IN)-infected mice. Intratracheal (IT) infection results in dissemination of the virus from the lungs to the brain and eyes via trigeminal and optic nerves. Ocular and neuronal invasions are confirmed using intracerebral (IC) infection. Notably, the eye-dropped (ED) virus does not cause lung infection and becomes undetectable with time. Ocular and neurotropic distribution of the virus in vivo is evident in fluorescence imaging with an infectious clone of SARS-CoV-2-mCherry. The ocular tropic and neuroinvasive characteristics of SARS-CoV-2 are confirmed in wild-type Syrian hamsters. Our data can improve the understanding regarding viral transmission and clinical characteristics of SARS-CoV-2 and help in improving COVID-19 control procedures.

Suggested Citation

  • Gi Uk Jeong & Hyung-Jun Kwon & Wern Hann Ng & Xiang Liu & Hyun Woo Moon & Gun Young Yoon & Hye Jin Shin & In-Chul Lee & Zheng Lung Ling & Alanna G. Spiteri & Nicholas J. C. King & Adam Taylor & Ji Soo, 2022. "Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35225-1
    DOI: 10.1038/s41467-022-35225-1
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    References listed on IDEAS

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    1. Wei Deng & Linlin Bao & Hong Gao & Zhiguang Xiang & Yajin Qu & Zhiqi Song & Shuran Gong & Jiayi Liu & Jiangning Liu & Pin Yu & Feifei Qi & Yanfeng Xu & Fengli Li & Chong Xiao & Qi Lv & Jing Xue & Qian, 2020. "Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    2. Sin Fun Sia & Li-Meng Yan & Alex W. H. Chin & Kevin Fung & Ka-Tim Choy & Alvina Y. L. Wong & Prathanporn Kaewpreedee & Ranawaka A. P. M. Perera & Leo L. M. Poon & John M. Nicholls & Malik Peiris & Hui, 2020. "Pathogenesis and transmission of SARS-CoV-2 in golden hamsters," Nature, Nature, vol. 583(7818), pages 834-838, July.
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