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Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection

Author

Listed:
  • Carlos Ávila-Nieto

    (Campus Can Ruti)

  • Júlia Vergara-Alert

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Pep Amengual-Rigo

    (Barcelona Supercomputing Center (BSC))

  • Erola Ainsua-Enrich

    (Campus Can Ruti)

  • Marco Brustolin

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Institute of Tropical Medicine)

  • María Luisa Rodríguez de la Concepción

    (Campus Can Ruti)

  • Núria Pedreño-Lopez

    (Campus Can Ruti)

  • Jordi Rodon

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Victor Urrea

    (Campus Can Ruti)

  • Edwards Pradenas

    (Campus Can Ruti)

  • Silvia Marfil

    (Campus Can Ruti)

  • Ester Ballana

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Eva Riveira-Muñoz

    (Campus Can Ruti)

  • Mònica Pérez

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Núria Roca

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Ferran Tarrés-Freixas

    (Campus Can Ruti
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Guillermo Cantero

    (Campus de la Universitat Autònoma de Barcelona (UAB))

  • Anna Pons-Grífols

    (Campus Can Ruti)

  • Carla Rovirosa

    (Campus Can Ruti)

  • Carmen Aguilar-Gurrieri

    (Campus Can Ruti)

  • Raquel Ortiz

    (Campus Can Ruti)

  • Ana Barajas

    (Campus Can Ruti)

  • Benjamin Trinité

    (Campus Can Ruti)

  • Rosalba Lepore

    (Barcelona Supercomputing Center (BSC))

  • Jordana Muñoz-Basagoiti

    (Campus Can Ruti)

  • Daniel Perez-Zsolt

    (Campus Can Ruti)

  • Nuria Izquierdo-Useros

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Alfonso Valencia

    (Barcelona Supercomputing Center (BSC)
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Julià Blanco

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Victor Guallar

    (Barcelona Supercomputing Center (BSC)
    Catalan Institution for Research and Advanced Studies)

  • Bonaventura Clotet

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Joaquim Segalés

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Universitat Autonoma de Barcelona. Bellaterra
    Bellaterra)

  • Jorge Carrillo

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

Abstract

Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunogenicity. However, S-2P is still produced at low levels. Here, we describe the V987H mutation that increases by two-fold the production of the recombinant Spike and the exposure of the receptor binding domain (RBD). S-V987H immunogenicity is similar to S-2P in mice and golden Syrian hamsters (GSH), and superior to a monomeric RBD. S-V987H immunization confer full protection against severe disease in K18-hACE2 mice and GSH upon SARS-CoV-2 challenge (D614G or B.1.351 variants). Furthermore, S-V987H immunized K18-hACE2 mice show a faster tissue viral clearance than RBD- or S-2P-vaccinated animals challenged with D614G, B.1.351 or Omicron BQ1.1 variants. Thus, S-V987H protein might be considered for future SARS-CoV-2 vaccines development.

Suggested Citation

  • Carlos Ávila-Nieto & Júlia Vergara-Alert & Pep Amengual-Rigo & Erola Ainsua-Enrich & Marco Brustolin & María Luisa Rodríguez de la Concepción & Núria Pedreño-Lopez & Jordi Rodon & Victor Urrea & Edwar, 2024. "Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46714-w
    DOI: 10.1038/s41467-024-46714-w
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