IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-46714-w.html
   My bibliography  Save this article

Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection

Author

Listed:
  • Carlos Ávila-Nieto

    (Campus Can Ruti)

  • Júlia Vergara-Alert

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Pep Amengual-Rigo

    (Barcelona Supercomputing Center (BSC))

  • Erola Ainsua-Enrich

    (Campus Can Ruti)

  • Marco Brustolin

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Institute of Tropical Medicine)

  • María Luisa Rodríguez de la Concepción

    (Campus Can Ruti)

  • Núria Pedreño-Lopez

    (Campus Can Ruti)

  • Jordi Rodon

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Victor Urrea

    (Campus Can Ruti)

  • Edwards Pradenas

    (Campus Can Ruti)

  • Silvia Marfil

    (Campus Can Ruti)

  • Ester Ballana

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Eva Riveira-Muñoz

    (Campus Can Ruti)

  • Mònica Pérez

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Núria Roca

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Ferran Tarrés-Freixas

    (Campus Can Ruti
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Guillermo Cantero

    (Campus de la Universitat Autònoma de Barcelona (UAB))

  • Anna Pons-Grífols

    (Campus Can Ruti)

  • Carla Rovirosa

    (Campus Can Ruti)

  • Carmen Aguilar-Gurrieri

    (Campus Can Ruti)

  • Raquel Ortiz

    (Campus Can Ruti)

  • Ana Barajas

    (Campus Can Ruti)

  • Benjamin Trinité

    (Campus Can Ruti)

  • Rosalba Lepore

    (Barcelona Supercomputing Center (BSC))

  • Jordana Muñoz-Basagoiti

    (Campus Can Ruti)

  • Daniel Perez-Zsolt

    (Campus Can Ruti)

  • Nuria Izquierdo-Useros

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Alfonso Valencia

    (Barcelona Supercomputing Center (BSC)
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Julià Blanco

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Victor Guallar

    (Barcelona Supercomputing Center (BSC)
    Catalan Institution for Research and Advanced Studies)

  • Bonaventura Clotet

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Joaquim Segalés

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Universitat Autonoma de Barcelona. Bellaterra
    Bellaterra)

  • Jorge Carrillo

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

Abstract

Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunogenicity. However, S-2P is still produced at low levels. Here, we describe the V987H mutation that increases by two-fold the production of the recombinant Spike and the exposure of the receptor binding domain (RBD). S-V987H immunogenicity is similar to S-2P in mice and golden Syrian hamsters (GSH), and superior to a monomeric RBD. S-V987H immunization confer full protection against severe disease in K18-hACE2 mice and GSH upon SARS-CoV-2 challenge (D614G or B.1.351 variants). Furthermore, S-V987H immunized K18-hACE2 mice show a faster tissue viral clearance than RBD- or S-2P-vaccinated animals challenged with D614G, B.1.351 or Omicron BQ1.1 variants. Thus, S-V987H protein might be considered for future SARS-CoV-2 vaccines development.

Suggested Citation

  • Carlos Ávila-Nieto & Júlia Vergara-Alert & Pep Amengual-Rigo & Erola Ainsua-Enrich & Marco Brustolin & María Luisa Rodríguez de la Concepción & Núria Pedreño-Lopez & Jordi Rodon & Victor Urrea & Edwar, 2024. "Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46714-w
    DOI: 10.1038/s41467-024-46714-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-46714-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-46714-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Weina Sun & Yonghong Liu & Fatima Amanat & Irene González-Domínguez & Stephen McCroskery & Stefan Slamanig & Lynda Coughlan & Victoria Rosado & Nicholas Lemus & Sonia Jangra & Raveen Rathnasinghe & Mi, 2021. "A Newcastle disease virus expressing a stabilized spike protein of SARS-CoV-2 induces protective immune responses," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Jarek Juraszek & Lucy Rutten & Sven Blokland & Pascale Bouchier & Richard Voorzaat & Tina Ritschel & Mark J. G. Bakkers & Ludovic L. R. Renault & Johannes P. M. Langedijk, 2021. "Stabilizing the closed SARS-CoV-2 spike trimer," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    3. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "Author Correction: A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 580(7803), pages 7-7, April.
    4. Kizzmekia S. Corbett & Darin K. Edwards & Sarah R. Leist & Olubukola M. Abiona & Seyhan Boyoglu-Barnum & Rebecca A. Gillespie & Sunny Himansu & Alexandra Schäfer & Cynthia T. Ziwawo & Anthony T. DiPia, 2020. "SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness," Nature, Nature, vol. 586(7830), pages 567-571, October.
    5. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 579(7798), pages 265-269, March.
    6. Florian Krammer, 2020. "SARS-CoV-2 vaccines in development," Nature, Nature, vol. 586(7830), pages 516-527, October.
    7. Sin Fun Sia & Li-Meng Yan & Alex W. H. Chin & Kevin Fung & Ka-Tim Choy & Alvina Y. L. Wong & Prathanporn Kaewpreedee & Ranawaka A. P. M. Perera & Leo L. M. Poon & John M. Nicholls & Malik Peiris & Hui, 2020. "Pathogenesis and transmission of SARS-CoV-2 in golden hamsters," Nature, Nature, vol. 583(7818), pages 834-838, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Timothy J. C. Tan & Zongjun Mou & Ruipeng Lei & Wenhao O. Ouyang & Meng Yuan & Ge Song & Raiees Andrabi & Ian A. Wilson & Collin Kieffer & Xinghong Dai & Kenneth A. Matreyek & Nicholas C. Wu, 2023. "High-throughput identification of prefusion-stabilizing mutations in SARS-CoV-2 spike," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Neeltje van Doremalen & Jonathan E. Schulz & Danielle R. Adney & Taylor A. Saturday & Robert J. Fischer & Claude Kwe Yinda & Nazia Thakur & Joseph Newman & Marta Ulaszewska & Sandra Belij-Rammerstorfe, 2022. "ChAdOx1 nCoV-19 (AZD1222) or nCoV-19-Beta (AZD2816) protect Syrian hamsters against Beta Delta and Omicron variants," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Phanramphoei N. Frantz & Aleksandr Barinov & Claude Ruffié & Chantal Combredet & Valérie Najburg & Guilherme Dias de Melo & Florence Larrous & Lauriane Kergoat & Samaporn Teeravechyan & Anan Jongkaeww, 2021. "A live measles-vectored COVID-19 vaccine induces strong immunity and protection from SARS-CoV-2 challenge in mice and hamsters," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    4. Dongsheng Chen & Jian Sun & Jiacheng Zhu & Xiangning Ding & Tianming Lan & Xiran Wang & Weiying Wu & Zhihua Ou & Linnan Zhu & Peiwen Ding & Haoyu Wang & Lihua Luo & Rong Xiang & Xiaoling Wang & Jiayin, 2021. "Single cell atlas for 11 non-model mammals, reptiles and birds," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    5. Irizar, Patricia & Kapadia, Dharmi & Amele, Sarah & Bécares, Laia & Divall, Pip & Katikireddi, Srinivasa Vittal & Kibuchi, Eliud & Kneale, Dylan & McCabe, Ronan & Nazroo, James & Nellums, Laura B. & T, 2023. "Pathways to ethnic inequalities in COVID-19 health outcomes in the United Kingdom: A systematic map," Social Science & Medicine, Elsevier, vol. 329(C).
    6. Mubango Hazel & Muzariri Calvin, 2022. "Employee Engagement and Competitive Advantage during Covid 19 Pandemic in Small to Medium Enterprises, Catering Industry, Harare," International Journal of Research and Innovation in Social Science, International Journal of Research and Innovation in Social Science (IJRISS), vol. 6(4), pages 288-292, April.
    7. Giulia Orilisi & Marco Mascitti & Lucrezia Togni & Riccardo Monterubbianesi & Vincenzo Tosco & Flavia Vitiello & Andrea Santarelli & Angelo Putignano & Giovanna Orsini, 2021. "Oral Manifestations of COVID-19 in Hospitalized Patients: A Systematic Review," IJERPH, MDPI, vol. 18(23), pages 1-19, November.
    8. David Gomez-Zepeda & Danielle Arnold-Schild & Julian Beyrle & Arthur Declercq & Ralf Gabriels & Elena Kumm & Annica Preikschat & Mateusz Krzysztof Łącki & Aurélie Hirschler & Jeewan Babu Rijal & Chris, 2024. "Thunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    9. Francesco Gangi & Eugenio D'Angelo & Lucia Michela Daniele & Nicola Varrone, 2021. "Assessing the impact of socially responsible human resources management on company environmental performance and cost of debt," Corporate Social Responsibility and Environmental Management, John Wiley & Sons, vol. 28(5), pages 1511-1527, September.
    10. Miquel Oliu-Barton & Bary S. R. Pradelski & Nicolas Woloszko & Lionel Guetta-Jeanrenaud & Philippe Aghion & Patrick Artus & Arnaud Fontanet & Philippe Martin & Guntram B. Wolff, 2022. "The effect of COVID certificates on vaccine uptake, health outcomes, and the economy," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    11. Sneha Gautam & Cyril Samuel & Alok Sagar Gautam & Sanjeev Kumar, 2021. "Strong link between coronavirus count and bad air: a case study of India," Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, Springer, vol. 23(11), pages 16632-16645, November.
    12. Hengrui Liu & Sho Iketani & Arie Zask & Nisha Khanizeman & Eva Bednarova & Farhad Forouhar & Brandon Fowler & Seo Jung Hong & Hiroshi Mohri & Manoj S. Nair & Yaoxing Huang & Nicholas E. S. Tay & Sumin, 2022. "Development of optimized drug-like small molecule inhibitors of the SARS-CoV-2 3CL protease for treatment of COVID-19," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    13. Michael Messer, 2022. "Bivariate change point detection: Joint detection of changes in expectation and variance," Scandinavian Journal of Statistics, Danish Society for Theoretical Statistics;Finnish Statistical Society;Norwegian Statistical Association;Swedish Statistical Association, vol. 49(2), pages 886-916, June.
    14. Alessandro Germani & Livia Buratta & Elisa Delvecchio & Claudia Mazzeschi, 2020. "Emerging Adults and COVID-19: The Role of Individualism-Collectivism on Perceived Risks and Psychological Maladjustment," IJERPH, MDPI, vol. 17(10), pages 1-15, May.
    15. Ioannis Kontoyiannis & Lambros Mertzanis & Athina Panotopoulou & Ioannis Papageorgiou & Maria Skoularidou, 2022. "Bayesian context trees: Modelling and exact inference for discrete time series," Journal of the Royal Statistical Society Series B, Royal Statistical Society, vol. 84(4), pages 1287-1323, September.
    16. Gabriela Dias Noske & Yun Song & Rafaela Sachetto Fernandes & Rod Chalk & Haitem Elmassoudi & Lizbé Koekemoer & C. David Owen & Tarick J. El-Baba & Carol V. Robinson & Glaucius Oliva & Andre Schutzer , 2023. "An in-solution snapshot of SARS-COV-2 main protease maturation process and inhibition," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    17. Eugene Song & Jae-Eun Lee & Seola Kwon, 2021. "Effect of Public Empathy with Infection-Control Guidelines on Infection-Prevention Attitudes and Behaviors: Based on the Case of COVID-19," IJERPH, MDPI, vol. 18(24), pages 1-18, December.
    18. Kow-Tong Chen, 2022. "Emerging Infectious Diseases and One Health: Implication for Public Health," IJERPH, MDPI, vol. 19(15), pages 1-4, July.
    19. Sui Zhang & Minghao Wang & Zhao Yang & Baolei Zhang, 2021. "A Novel Predictor for Micro-Scale COVID-19 Risk Modeling: An Empirical Study from a Spatiotemporal Perspective," IJERPH, MDPI, vol. 18(24), pages 1-16, December.
    20. Shujuan Li & Lingli Zhu & Lidan Zhang & Guoyan Zhang & Hongyan Ren & Liang Lu, 2023. "Urbanization-Related Environmental Factors and Hemorrhagic Fever with Renal Syndrome: A Review Based on Studies Taken in China," IJERPH, MDPI, vol. 20(4), pages 1-20, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46714-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.