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The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Author

Listed:
  • Hui Wang

    (Peking University
    Chengdu Medical College)

  • Boyuan Li

    (Peking University)

  • Linyu Zuo

    (Peking University)

  • Bo Wang

    (Peking University)

  • Yan Yan

    (Tsinghua University
    Tsinghua University)

  • Kai Tian

    (Peking University)

  • Rong Zhou

    (Peking University)

  • Chenlu Wang

    (Peking University)

  • Xizi Chen

    (Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University)

  • Yongpeng Jiang

    (Peking University)

  • Haonan Zheng

    (Peking University)

  • Fangfei Qin

    (Peking University)

  • Bin Zhang

    (University of Rochester Medical Center)

  • Yang Yu

    (Chinese Academy of Sciences)

  • Chao-Pei Liu

    (Chinese Academy of Sciences)

  • Yanhui Xu

    (Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University)

  • Juntao Gao

    (Tsinghua University
    Tsinghua University)

  • Zhi Qi

    (Peking University)

  • Wulan Deng

    (Peking University)

  • Xiong Ji

    (Peking University)

Abstract

RNA polymerase II (Pol II) apparatuses are compartmentalized into transcriptional clusters. Whether protein factors control these clusters remains unknown. In this study, we find that the ATPase-associated with diverse cellular activities (AAA + ) ATPase RUVBL2 co-occupies promoters with Pol II and various transcription factors. RUVBL2 interacts with unphosphorylated Pol II in chromatin to promote RPB1 carboxy-terminal domain (CTD) clustering and transcription initiation. Rapid depletion of RUVBL2 leads to a decrease in the number of Pol II clusters and inhibits nascent RNA synthesis, and tethering RUVBL2 to an active promoter enhances Pol II clustering at the promoter. We also identify target genes that are directly linked to the RUVBL2-Pol II axis. Many of these genes are hallmarks of cancers and encode proteins with diverse cellular functions. Our results demonstrate an emerging activity for RUVBL2 in regulating Pol II cluster formation in the nucleus.

Suggested Citation

  • Hui Wang & Boyuan Li & Linyu Zuo & Bo Wang & Yan Yan & Kai Tian & Rong Zhou & Chenlu Wang & Xizi Chen & Yongpeng Jiang & Haonan Zheng & Fangfei Qin & Bin Zhang & Yang Yu & Chao-Pei Liu & Yanhui Xu & J, 2022. "The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors," Nature Communications, Nature, vol. 13(1), pages 1-26, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33433-3
    DOI: 10.1038/s41467-022-33433-3
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    References listed on IDEAS

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    1. Yu-Fei Cao & Hui Wang & Yong Sun & Bei-Bei Tong & Wen-Qi Shi & Liu Peng & Yi-Meng Zhang & Yu-Qiu Wu & Teng Fu & Hua-Yan Zou & Kai Zhang & Li-Yan Xu & En-Min Li, 2025. "Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    2. Mingming Xing & Yang Li & Yuqi Zhang & Juemou Zhou & Danting Ma & Mengqi Zhang & Minglei Tang & Ting Ouyang & Fumiao Zhang & Xiaofeng Shi & Jianyuan Sun & Zuxin Chen & Weiping J. Zhang & Shuli Zhang &, 2024. "Paraventricular hypothalamic RUVBL2 neurons suppress appetite by enhancing excitatory synaptic transmission in distinct neurocircuits," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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