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Stoichiometry and turnover in single, functioning membrane protein complexes

Author

Listed:
  • Mark C. Leake

    (University of Oxford)

  • Jennifer H. Chandler

    (University of Oxford)

  • George H. Wadhams

    (University of Oxford)

  • Fan Bai

    (University of Oxford)

  • Richard M. Berry

    (University of Oxford)

  • Judith P. Armitage

    (University of Oxford)

Abstract

The dynamic exchange of motor proteins in a functioning 45-nm rotary complex was observed in vivo and GFP–MotB stator proteins visualized in individual bacterial flagellar motors. Fluorescence recovery after photobleaching reveals that the ∼22 MotB motor proteins exchange rapidly with a pool of free protein while the motor is actively rotating.

Suggested Citation

  • Mark C. Leake & Jennifer H. Chandler & George H. Wadhams & Fan Bai & Richard M. Berry & Judith P. Armitage, 2006. "Stoichiometry and turnover in single, functioning membrane protein complexes," Nature, Nature, vol. 443(7109), pages 355-358, September.
    • Handle: RePEc:nat:nature:v:443:y:2006:i:7109:d:10.1038_nature05135
    DOI: 10.1038/nature05135
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    Cited by:

    1. Basila Moochickal Assainar & Kaushik Ragunathan & Ryan D. Baldridge, 2024. "Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Haidai Hu & Philipp F. Popp & Mònica Santiveri & Aritz Roa-Eguiara & Yumeng Yan & Freddie J. O. Martin & Zheyi Liu & Navish Wadhwa & Yong Wang & Marc Erhardt & Nicholas M. I. Taylor, 2023. "Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Chenrui Qin & Yanhui Xiang & Jie Liu & Ruilin Zhang & Ziming Liu & Tingting Li & Zhi Sun & Xiaoyi Ouyang & Yeqing Zong & Haoqian M. Zhang & Qi Ouyang & Long Qian & Chunbo Lou, 2023. "Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    4. Jack W. Shepherd & Sebastien Guilbaud & Zhaokun Zhou & Jamieson A. L. Howard & Matthew Burman & Charley Schaefer & Adam Kerrigan & Clare Steele-King & Agnes Noy & Mark C. Leake, 2024. "Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Navish Wadhwa & Alberto Sassi & Howard C. Berg & Yuhai Tu, 2022. "A multi-state dynamic process confers mechano-adaptation to a biological nanomachine," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    6. Yoriko Lill & Lorne D Jordan & Chuck R Smallwood & Salete M Newton & Markus A Lill & Phillip E Klebba & Ken Ritchie, 2016. "Confined Mobility of TonB and FepA in Escherichia coli Membranes," PLOS ONE, Public Library of Science, vol. 11(12), pages 1-18, December.
    7. Cyril F Reboul & Daniel A Andrews & Musammat F Nahar & Ashley M Buckle & Anna Roujeinikova, 2011. "Crystallographic and Molecular Dynamics Analysis of Loop Motions Unmasking the Peptidoglycan-Binding Site in Stator Protein MotB of Flagellar Motor," PLOS ONE, Public Library of Science, vol. 6(4), pages 1-7, April.

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