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Tension directly stabilizes reconstituted kinetochore-microtubule attachments

Author

Listed:
  • Bungo Akiyoshi

    (Fred Hutchinson Cancer Research Center
    Molecular and Cellular Biology Program, University of Washington)

  • Krishna K. Sarangapani

    (University of Washington)

  • Andrew F. Powers

    (University of Washington)

  • Christian R. Nelson

    (Fred Hutchinson Cancer Research Center)

  • Steve L. Reichow

    (University of Washington)

  • Hugo Arellano-Santoyo

    (Fred Hutchinson Cancer Research Center
    Molecular and Cellular Biology Program, University of Washington
    University of Washington)

  • Tamir Gonen

    (University of Washington
    Howard Hughes Medical Institute, University of Washington)

  • Jeffrey A. Ranish

    (Institute for Systems Biology)

  • Charles L. Asbury

    (University of Washington)

  • Sue Biggins

    (Fred Hutchinson Cancer Research Center)

Abstract

Stability of kinetochore–microtubule binding The kinetochore is the large protein complex that assembles on centromeric DNA to mediate chromosome separation. For decades, researchers have tried to isolate whole functional kinetochores without success. Sue Biggins and colleagues now report the first purification of functional kinetochores. They also show that kinetochore particles maintain load-bearing associations with assembling and disassembling microtubules, and that tension directly increases the lifetimes of the attachments. These results provide evidence that tension selectively stabilizes kinetochore–microtubule interactions.

Suggested Citation

  • Bungo Akiyoshi & Krishna K. Sarangapani & Andrew F. Powers & Christian R. Nelson & Steve L. Reichow & Hugo Arellano-Santoyo & Tamir Gonen & Jeffrey A. Ranish & Charles L. Asbury & Sue Biggins, 2010. "Tension directly stabilizes reconstituted kinetochore-microtubule attachments," Nature, Nature, vol. 468(7323), pages 576-579, November.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7323:d:10.1038_nature09594
    DOI: 10.1038/nature09594
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    Citations

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    Cited by:

    1. Anna K. Regt & Cordell J. Clark & Charles L. Asbury & Sue Biggins, 2022. "Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Navish Wadhwa & Alberto Sassi & Howard C. Berg & Yuhai Tu, 2022. "A multi-state dynamic process confers mechano-adaptation to a biological nanomachine," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    3. Ishutesh Jain & Mandar M Inamdar & Ranjith Padinhateeri, 2015. "Statistical Mechanics Provides Novel Insights into Microtubule Stability and Mechanism of Shrinkage," PLOS Computational Biology, Public Library of Science, vol. 11(2), pages 1-23, February.
    4. Hyun-Kyu Choi & Peiwen Cong & Chenghao Ge & Aswin Natarajan & Baoyu Liu & Yong Zhang & Kaitao Li & Muaz Nik Rushdi & Wei Chen & Jizhong Lou & Michelle Krogsgaard & Cheng Zhu, 2023. "Catch bond models may explain how force amplifies TCR signaling and antigen discrimination," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    5. Lee-Ya Chu & Daniel Stedman & Julian Gannon & Susan Cox & Georgii Pobegalov & Maxim I. Molodtsov, 2024. "Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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