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Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens

Author

Listed:
  • Irene Serra

    (Instituto Cajal, CSIC)

  • Julio Esparza

    (Instituto Cajal, CSIC)

  • Laura Delgado

    (Instituto Cajal, CSIC)

  • Cristina Martín-Monteagudo

    (Instituto Cajal, CSIC)

  • Margalida Puigròs

    (Institute for Biomedical Research of Barcelona, CSIC, CIBERNED)

  • Petar Podlesniy

    (Institute for Biomedical Research of Barcelona, CSIC, CIBERNED)

  • Ramón Trullás

    (Institute for Biomedical Research of Barcelona, CSIC, CIBERNED)

  • Marta Navarrete

    (Instituto Cajal, CSIC)

Abstract

Astrocytes are key elements of brain circuits that are involved in different aspects of the neuronal physiology relevant to brain functions. Although much effort is being made to understand how the biology of astrocytes affects brain circuits, astrocytic network heterogeneity and plasticity is still poorly defined. Here, we have combined structural and functional imaging of astrocyte activity recorded in mice using the Ca2+-modulated photoactivatable ratiometric integrator and specific optostimulation of glutamatergic pathways to map the functional neuron-astrocyte circuitries in the nucleus accumbens (NAc). We showed pathway-specific astrocytic responses induced by selective optostimulation of main inputs from the prefrontal cortex, basolateral amygdala, and ventral hippocampus. Furthermore, co-stimulation of glutamatergic pathways induced non-linear Ca2+-signaling integration, revealing integrative properties of NAc astrocytes. All these results demonstrate the existence of specific neuron-astrocyte circuits in the NAc, providing an insight to the understanding of how the NAc integrates information.

Suggested Citation

  • Irene Serra & Julio Esparza & Laura Delgado & Cristina Martín-Monteagudo & Margalida Puigròs & Petar Podlesniy & Ramón Trullás & Marta Navarrete, 2022. "Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33020-6
    DOI: 10.1038/s41467-022-33020-6
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