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Using antibodies to control DNA-templated chemical reactions

Author

Listed:
  • Lorena Baranda Pellejero

    (University of Rome, Tor Vergata, Via della Ricerca Scientifica)

  • Malihe Mahdifar

    (University of Rome, Tor Vergata, Via della Ricerca Scientifica)

  • Gianfranco Ercolani

    (University of Rome, Tor Vergata, Via della Ricerca Scientifica)

  • Jonathan Watson

    (ATDBio Ltd, Magdalen Centre, Oxford Science Park)

  • Tom Brown

    (ATDBio Ltd, Magdalen Centre, Oxford Science Park)

  • Francesco Ricci

    (University of Rome, Tor Vergata, Via della Ricerca Scientifica)

Abstract

DNA-templated synthesis takes advantage of the programmability of DNA-DNA interactions to accelerate chemical reactions under diluted conditions upon sequence-specific hybridization. While this strategy has proven advantageous for a variety of applications, including sensing and drug discovery, it has been so far limited to the use of nucleic acids as templating elements. Here, we report the rational design of DNA templated synthesis controlled by specific IgG antibodies. Our approach is based on the co-localization of reactants induced by the bivalent binding of a specific IgG antibody to two antigen-conjugated DNA templating strands that triggers a chemical reaction that would be otherwise too slow under diluted conditions. This strategy is versatile, orthogonal and adaptable to different IgG antibodies and can be employed to achieve the targeted synthesis of clinically-relevant molecules in the presence of specific IgG biomarker antibodies.

Suggested Citation

  • Lorena Baranda Pellejero & Malihe Mahdifar & Gianfranco Ercolani & Jonathan Watson & Tom Brown & Francesco Ricci, 2020. "Using antibodies to control DNA-templated chemical reactions," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-20024-3
    DOI: 10.1038/s41467-020-20024-3
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    Cited by:

    1. Avik Samanta & Maximilian Hörner & Wei Liu & Wilfried Weber & Andreas Walther, 2022. "Signal-processing and adaptive prototissue formation in metabolic DNA protocells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Yuan Liang & Yunkai Qie & Jing Yang & Ranfeng Wu & Shuang Cui & Yuliang Zhao & Greg J. Anderson & Guangjun Nie & Suping Li & Cheng Zhang, 2023. "Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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